Artículo
Increased nitration and diminished activity of copper/zinc superoxide dismutase in placentas from diabetic rats
White, Verónica
; Capobianco, Evangelina Lorena
; Higa, Romina Daniela
; Martinez, Nora Alicia
; Sosa, María; Jawerbaum, Alicia Sandra
Fecha de publicación:
12/2010
Editorial:
Taylor & Francis
Revista:
Free Radical Research
ISSN:
1071-5762
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Nitration-induced protein damage in the placenta leads to impaired blood flow and deficient feto–placental exchange in diabetic pregnancies. This work studied the effect of nitric oxide and peroxynitrite on Cu/Zn SOD activity in rat placentas and evaluated whether Cu/Zn SOD is nitrated in the placenta from diabetic rats at mid-gestation. Protein nitration was evaluated by EIA, Cu/Zn SOD activity by inhibition of the epinephrine auto-oxidation, Cu/Zn SOD expression by western blot and specific nitration by immunoprecipitation. This study found higher levels of protein nitration (p < 0.001), diminished Cu/Zn SOD activity and enhanced protein expression (p < 0.01) in placentas from diabetic rats. Placental Cu/Zn SOD activity was inhibited by peroxynitrite (p < 0.01). Besides, nitration of Cu/Zn SOD was elevated in placentas from diabetic rats (p < 0.01). These results show that rat Cu/Zn SOD can be nitrated, a modification that could lead to the depressed activity of this enzyme found in placentas from diabetic rats.
Palabras clave:
Placenta
,
Sod
,
Peroxynitrite
,
Nitrotyrosine
,
Cu/Zn
,
Diabetes
,
Rat
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CEFYBO)
Articulos de CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS
Articulos de CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS
Citación
White, Verónica; Capobianco, Evangelina Lorena; Higa, Romina Daniela; Martinez, Nora Alicia; Sosa, María; et al.; Increased nitration and diminished activity of copper/zinc superoxide dismutase in placentas from diabetic rats; Taylor & Francis; Free Radical Research; 44; 12; 12-2010; 1407-1415
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