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dc.contributor.author
Gomez Palacios, Luna R.
dc.contributor.author
Veglia, Alicia Viviana
dc.contributor.author
Bracamonte, Angel Guillermo
dc.date.available
2022-10-11T16:31:35Z
dc.date.issued
2021-10
dc.identifier.citation
Gomez Palacios, Luna R.; Veglia, Alicia Viviana; Bracamonte, Angel Guillermo; Inflow nano-optics from the near-to the far-field detection based on Metal-Enhanced Fluorescence signaling; Elsevier Science; Microchemical Journal; 169; 1065; 10-2021; 1-9
dc.identifier.issn
0026-265X
dc.identifier.uri
http://hdl.handle.net/11336/172525
dc.description.abstract
In this communication we evaluated the detection and tracking of Ultraluminescent gold core–shell nanoplatforms based on Metal-Enhanced Fluorescence (MEF) within colloidal dispersion by In-Flow Cytometry and Imaging Flow Cytometry (IFC). The enhanced properties were developed by the incorporation of Rhodamine B (RhB) as a laser dye reporter at variable controlled silica spacer lengths (Au@SiO2-RhB) from a gold core template. Thus, SiO2 was varied from 6 to 7 nm to 25 nm in order to place the laser dye sensitizer at different lengths of the higher electromagnetic fields surrounding the 45-nm gold core diameter. By Laser Fluorescence Microscopy, high intense emissions were shown at shorter SiO2 lengths, while lower emissions were found towards longer ones. These characteristics related to MEF were evaluated by in-flow cytometry with dual laser excitation and fluorescence detection, and by an IFC set-up. By In-flow cytometry, different scattered-light distributions were shown, depending on the nanoparticle concentrations in the colloidal dispersion and size of the Au@SiO2-RhB. Moreover, varied fluorescent event detections and counting were recorded according to the SiO2 lengths applied. A ratio of fluorescent event detection counting of core–shell/core-less of 40–42 was established for optimal MEF Enhancement Factors (MEFEF) of 41–43 determined by Laser Fluorescence Microscopy. Core-less nanoparticles showed a marked decrease of fluorescent event detection counting caused by higher photobleaching and absence of the MEF effect. Hence, an improved detection effect was shown within in-flow based on MEF from single nanoplatforms. Therefore, the Ultraluminescence emission of core–shell nanoarchitectures within in-flow techniques was shown and discussed. A non-classical light in real time within colloidal gold core–shell nanoparticles was generated by accurate control of their architectures.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
IN-FLOW NANO-EMITTER DETECTION
dc.subject
IN-FLOW NANOPARTICLE CHARACTERIZATION
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IN-FLOW METAL-ENHANCED FLUORESCENCE SIGNALING
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METAL.ENHANCED FLUORESCENCE
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GOLD CORE-SHELL NANOPARTICLE
dc.subject.classification
Química Coloidal
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Ciencias Químicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Inflow nano-optics from the near-to the far-field detection based on Metal-Enhanced Fluorescence signaling
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-09-21T23:24:33Z
dc.journal.volume
169
dc.journal.number
1065
dc.journal.pagination
1-9
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Gomez Palacios, Luna R.. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
dc.description.fil
Fil: Veglia, Alicia Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
dc.description.fil
Fil: Bracamonte, Angel Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina. Laval University; Canadá
dc.journal.title
Microchemical Journal
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0026265X21006251
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.microc.2021.106539
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