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dc.contributor.author
Huarte, Hector Roberto
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dc.contributor.author
Puglia, Giuseppe. D.
dc.contributor.author
Prjibelski, Andrey D.
dc.contributor.author
Raccuia, Salvatore A.
dc.date.available
2022-10-03T14:21:43Z
dc.date.issued
2020-09
dc.identifier.citation
Huarte, Hector Roberto; Puglia, Giuseppe. D.; Prjibelski, Andrey D.; Raccuia, Salvatore A.; Seed transcriptome annotation reveals enhanced expression of genes related to ros homeostasis and ethylene metabolism at alternating temperatures in wild cardoon; MDPI AG; Plants; 9; 9; 9-2020; 1-19
dc.identifier.issn
2223-7747
dc.identifier.uri
http://hdl.handle.net/11336/171449
dc.description.abstract
The association among environmental cues, ethylene response, ABA signaling, and reactive oxygen species (ROS) homeostasis in the process of seed dormancy release is nowadays well-established in many species. Alternating temperatures are recognized as one of the main environmental signals determining dormancy release, but their underlying mechanisms are scarcely known. Dry after-ripened wild cardoon achenes germinated poorly at a constant temperature of 20, 15, or 10◦C, whereas germination was stimulated by 80% at alternating temperatures of 20/10◦C. Using an RNA-Seq approach, we identified 23,640 and annotated 14,078 gene transcripts expressed in dry achenes and achenes exposed to constant or alternating temperatures. Transcriptional patterns identified in dry condition included seed reserve and response to dehydration stress genes (i.e., HSPs, peroxidases, and LEAs). At a constant temperature, we observed an upregulation of ABA biosynthesis genes (i.e., NCED9), ABA-responsive genes (i.e., ABI5 and TAP), as well as other genes previously related to physiological dormancy and inhibition of germination. However, the alternating temperatures were associated with the upregulation of ethylene metabolism (i.e., ACO1, 4, and ACS10) and signaling (i.e., EXPs) genes and ROS homeostasis regulators genes (i.e., RBOH and CAT). Accordingly, the ethylene production was twice as high at alternating than at constant temperatures. The presence in the germination medium of ethylene or ROS synthesis and signaling inhibitors reduced significantly, but not completely, germination at 20/10◦C. Conversely, the presence of methyl viologen and salicylhydroxamic acid (SHAM), a peroxidase inhibitor, partially increased germination at constant temperature. Taken together, the present study provides the first insights into the gene expression patterns and physiological response associated with dormancy release at alternating temperatures in wild cardoon (Cynara cardunculus var. sylvestris).
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
MDPI AG
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
ANTIOXIDANTS
dc.subject
DORMANCY TERMINATION
dc.subject
ENVIRONMENTAL SIGNALS
dc.subject
ETHYLENE SIGNALING
dc.subject
GENE EXPRESSION
dc.subject
RNA-SEQ
dc.subject.classification
Otras Ciencias Agrícolas
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dc.subject.classification
Otras Ciencias Agrícolas
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CIENCIAS AGRÍCOLAS
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dc.title
Seed transcriptome annotation reveals enhanced expression of genes related to ros homeostasis and ethylene metabolism at alternating temperatures in wild cardoon
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-09-26T17:51:02Z
dc.journal.volume
9
dc.journal.number
9
dc.journal.pagination
1-19
dc.journal.pais
Suiza
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dc.description.fil
Fil: Huarte, Hector Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Lomas de Zamora; Argentina
dc.description.fil
Fil: Puglia, Giuseppe. D.. Consiglio Nazionale delle Ricerche; Italia
dc.description.fil
Fil: Prjibelski, Andrey D.. Academia de Ciencias de Rusia.; Rusia
dc.description.fil
Fil: Raccuia, Salvatore A.. Consiglio Nazionale delle Ricerche; Italia
dc.journal.title
Plants
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2223-7747/9/9/1225
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3390/plants9091225
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