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dc.contributor.author
Barrantes, Francisco Jose
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dc.date.available
2022-09-28T15:23:43Z
dc.date.issued
2021-10
dc.identifier.citation
Barrantes, Francisco Jose; Fluorescence sensors for imaging membrane lipid domains and cholesterol; Elsevier Academic Press Inc.; Current Topics In Membranes; 88; 10-2021; 257-314
dc.identifier.issn
1063-5823
dc.identifier.uri
http://hdl.handle.net/11336/170769
dc.description.abstract
Lipid membrane domains are supramolecular lateral heterogeneities of biological membranes. Of nanoscopic dimensions, they constitute specialized hubs used by the cell as transient signaling platforms for a great variety of biologically important mechanisms. Their property to form and dissolve in the bulk lipid bilayer endow them with the ability to engage in highly dynamic processes, and temporarily recruit subpopulations of membrane proteins in reduced nanometric compartments that can coalesce to form larger mesoscale assemblies. Cholesterol is an essential component of these lipid domains; its unique molecular structure is suitable for interacting intricately with crevices and cavities of transmembrane protein surfaces through its rough β face while “talking” to fatty acid acyl chains of glycerophospholipids and sphingolipids via its smooth α face. Progress in the field of membrane domains has been closely associated with innovative improvements in fluorescence microscopy and new fluorescence sensors. These advances enabled the exploration of the biophysical properties of lipids and their supramolecular platforms. Here I review the rationale behind the use of biosensors over the last few decades and their contributions towards elucidation of the in-plane and transbilayer topography of cholesterol-enriched lipid domains and their molecular constituents. The challenges introduced by super-resolution optical microscopy are discussed, as well as possible scenarios for future developments in the field, including virtual (“no staining”) staining.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Academic Press Inc.
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dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
BIOSENSORS
dc.subject
CHOLESTEROL
dc.subject
FLUORESCENCE MICROSCOPY
dc.subject
FLUORESCENT PROBES
dc.subject
IN-PLANE DISTRIBUTION
dc.subject
LIPID ASYMMETRY
dc.subject
LIPID DOMAIN
dc.subject
MEMBRANE DOMAINS
dc.subject
NANOSCOPY
dc.subject
SENSORS
dc.subject
SUPER-RESOLUTION MICROSCOPY
dc.subject
TRANSBILAYER DISTRIBUTION
dc.subject
VIRTUAL STAINING
dc.subject.classification
Biofísica
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Fluorescence sensors for imaging membrane lipid domains and cholesterol
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-09-09T17:22:00Z
dc.journal.volume
88
dc.journal.pagination
257-314
dc.journal.pais
Estados Unidos
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dc.description.fil
Fil: Barrantes, Francisco Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
dc.journal.title
Current Topics In Membranes
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/bs.ctm.2021.09.004
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S1063582321000132?via%3Dihub
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