Artículo
SLAMF1 signaling induces Mycobacterium tuberculosis uptake leading to endolysosomal maturation in human macrophages
Barbero, Angela Maria
; Trotta, Aldana
; Genoula, Melanie
; Hernández del Pino, Rodrigo Emanuel
; Estermann, Martín Andrés; Celano, Josefina; Fuentes, Federico
; García, Verónica Edith
; Balboa, Luciana
; Barrionuevo, Paula
; Pasquinelli, Virginia
Fecha de publicación:
01/2021
Editorial:
Federation of American Societies for Experimental Biology
Revista:
Journal of Leukocyte Biology
ISSN:
0741-5400
e-ISSN:
1938-3673
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Tuberculosis dates back to ancient times but it is not a problem of the past. Each year, millions of people die from tuberculosis. After inhalation of infectious droplet nuclei, Mycobacterium tuberculosis reaches the lungs where it can manipulate the immune system and survive within host macrophages, establishing a persistent infection. The signaling lymphocytic activation molecule family member 1 (SLAMF1) is a self-ligand receptor that can internalize gram-negative bacteria and regulate macrophages’ phagosomal functions. In tuberculosis, SLAMF1 promotes Th1-protective responses. In this work, we studied the role of SLAMF1 on macrophages’ functions during M. tuberculosis infection. Our results showed that both M. tuberculosis and IFN-γ stimulation induce SLAMF1 expression in macrophages from healthy donor and Tohoku Hospital Pediatrcs-1 cells. Costimulation through SLAMF1 with an agonistic antibody resulted in an enhanced internalization of M. tuberculosis by macrophages. Interestingly, we found that SLAMF1 interacts with M. tuberculosis and colocalizes with the bacteria and with early and late endosomes/lysosomes markers (EEA1 and LAMP2), suggesting that SLAMF1 recognize M. tuberculosis and participate in the endolysosomal maturation process. Notably, increased levels of SLAMF1 were detected in CD14 cells from pleural effusions of tuberculosis patients, indicating that SLAMF1 might have an active function at the site of infection. Taken together, our results provide evidence that SLAMF1 improves the uptake of M. tuberculosis by human monocyte-derived macrophages.
Palabras clave:
ENDOCYTOSIS
,
MICROBIAL SENSOR
,
TUBERCULOSIS
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Articulos de SEDE CENTRAL
Citación
Barbero, Angela Maria; Trotta, Aldana; Genoula, Melanie; Hernández del Pino, Rodrigo Emanuel; Estermann, Martín Andrés; et al.; SLAMF1 signaling induces Mycobacterium tuberculosis uptake leading to endolysosomal maturation in human macrophages; Federation of American Societies for Experimental Biology; Journal of Leukocyte Biology; 109; 1; 1-2021; 257-273
Compartir
Altmétricas