Artículo
Genetic identification and drug-resistance characterization of mycobacterium tuberculosis using a portable sequencing device. A pilot study
Fecha de publicación:
09/2020
Editorial:
MDPI AG
Revista:
Antibiotics
ISSN:
2079-6382
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Clinical management of tuberculosis (TB) in endemic areas is often challenged by a lack of resources including laboratories for Mycobacterium tuberculosis (Mtb) culture. Traditional phenotypic drug susceptibility testing for Mtb is costly and time consuming, while PCR-based methods are limited to selected target loci. We herein utilized a portable, USB-powered, long-read sequencing instrument (MinION), to investigate Mtb genomic DNA from clinical isolates to determine the presence of anti-TB drug-resistance conferring mutations. Data analysis platform EPI2ME and antibiotic-resistance analysis using the real time ARMA workflow, identified Mtb species as well as extensive resistance gene profiles. The approach was highly sensitive, being able to detect almost all described drug resistance conferring mutations based on previous whole genome sequencing analysis. Our findings are supportive of the practical use of this system as a suitable method for the detection of antimicrobial resistance genes, and effective in providing Mtb genomic information. Future improvements in the error rate through statistical analysis, drug resistance prediction algorithms and reference databases would make this a platform suited for the clinical setting. The small size, relatively inexpensive cost of the device, as well as its rapid and simple library preparation protocol and analysis, make it an attractive option for settings with limited laboratory infrastructure.
Palabras clave:
DRUG RESISTANCE
,
MINION
,
NEXT-GENERATION SEQUENCING
,
TUBERCULOSIS
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Articulos de SEDE CENTRAL
Citación
Cervantes, Jorge; Yokobori, Noemí; Hong, Bo Young; Genetic identification and drug-resistance characterization of mycobacterium tuberculosis using a portable sequencing device. A pilot study; MDPI AG; Antibiotics; 9; 9; 9-2020; 1-15
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