Artículo
Control of MT1-MMP transport by atypical PKC during breast-cancer progression
Rossé, Carine; Lodillinsky, Catalina
; Fuhrmann, Laetitia; Nourieh, Maya; Monteiro, Pedro; Irondelle, Marie; Lagoutte, Emilie; Vacher, Sophie; Waharte, François; Paul Gilloteaux, Perrine; Romao, Maryse; Sengmanivong, Lucie; Linch, Mark; Van Lint, Johan; Raposo, Graça; Vincent Salomon, Anne; Bièche, Ivan; Parker, Peter J.; Chavrier, Philippe
Fecha de publicación:
05/2014
Editorial:
National Academy of Sciences
Revista:
Proceedings of the National Academy of Sciences of The United States of America
ISSN:
0027-8424
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Dissemination of carcinoma cells requires the pericellular degradation of the extracellular matrix, which is mediated by membrane type 1-matrix metalloproteinase (MT1-MMP). In this article, we report a co–up-regulation and colocalization of MT1-MMP and atypical protein kinase C iota (aPKCι) in hormone receptor-negative breast tumors in association with a higher risk of metastasis. Silencing of aPKC in invasive breast-tumor cell lines impaired the delivery of MT1-MMP from late endocytic storage compartments to the surface and inhibited matrix degradation and invasion. We provide evidence that aPKCι, in association with MT1-MMP–containing endosomes, phosphorylates cortactin, which is present in F-actin–rich puncta on MT1-MMP–positive endosomes and regulates cortactin association with the membrane scission protein dynamin-2. Thus, cell line-based observations and clinical data reveal the concerted activity of aPKC, cortactin, and dynamin-2, which control the trafficking of MT1-MMP from late endosome to the plasma membrane and play an important role in the invasive potential of breast-cancer cells.
membrane traffic actin cytoskeleton multi-vesicular body MMP14
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Articulos(OCA HOUSSAY)
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Citación
Rossé, Carine; Lodillinsky, Catalina; Fuhrmann, Laetitia; Nourieh, Maya; Monteiro, Pedro; et al.; Control of MT1-MMP transport by atypical PKC during breast-cancer progression; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 111; 18; 5-2014; 1872-1879
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