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dc.contributor.author
Gabay, Gilad
dc.contributor.author
Zhang, Junli
dc.contributor.author
Burguener, German Federico
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Howell, Tyson
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Wang, Hanchao
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Fahima, Tzion
dc.contributor.author
Lukaszewski, Adam
dc.contributor.author
Moriconi, Jorge Ignacio
dc.contributor.author
Santa Maria, Guillermo Esteban
dc.contributor.author
Dubcovsky, Jorge
dc.date.available
2022-09-21T17:01:20Z
dc.date.issued
2020-11
dc.identifier.citation
Gabay, Gilad; Zhang, Junli; Burguener, German Federico; Howell, Tyson; Wang, Hanchao; et al.; Structural rearrangements in wheat (1BS)–rye (1RS) recombinant chromosomes affect gene dosage and root length; John Wiley & Sons Inc.; Plant Genome; 11-2020; 1-16
dc.identifier.issn
1940-3372
dc.identifier.uri
http://hdl.handle.net/11336/169779
dc.description.abstract
Good understanding of the genes controlling root development is required to engineer root systems better adapted to different soil types. In wheat (Triticum aestivum L.), the 1RS.1BL wheat–rye (Secale cereale L.) translocation has been associated with improved drought tolerance and a large root system. However, an isogenic line carrying an interstitial segment from wheat chromosome arm 1BS in the distal region of the 1RS arm (1RSRW) showed reduced grain yield and shorter roots both in the field and in hydroponic cultures relative to isogenic lines with the complete 1RS arm. In this study, we used exome capture to characterize 1RSRW and its parental lines T-9 and 1B+40. We show that 1RSRW has a 7.0 Mb duplicated 1RS region and a 4.8 Mb 1BS insertion colinear with the 1RS duplication, resulting in triplicated genes. Lines homozygous for 1RSRW have short seminal roots, while lines heterozygous for this chromosome have roots of intermediate length. By contrast, near-isogenic lines carrying only the 1BS distal region or the 1RS-1BS duplication have long seminal roots similar to 1RS, suggesting a limited effect of the 1BS genes. These results suggest that the dosage of duplicated 1RS genes is critical for seminal root length. An induced deletion encompassing 38 orthologous wheat and rye duplicated genes restored root length and confirmed the importance of gene dosage in the short-root phenotype. We explored the expression profiles and functional annotation of these genes and discuss their potential as candidate genes for the regulation of seminal root length in wheat.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
John Wiley & Sons Inc.
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Wheat
dc.subject
Rye
dc.subject
1BS
dc.subject
1RS translocation
dc.subject.classification
Biotecnología Agrícola y Biotecnología Alimentaria
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Biotecnología Agropecuaria
dc.subject.classification
CIENCIAS AGRÍCOLAS
dc.title
Structural rearrangements in wheat (1BS)–rye (1RS) recombinant chromosomes affect gene dosage and root length
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-09-16T20:45:08Z
dc.journal.pagination
1-16
dc.journal.pais
Estados Unidos
dc.description.fil
Fil: Gabay, Gilad. University of California at Davis; Estados Unidos
dc.description.fil
Fil: Zhang, Junli. University of California at Davis; Estados Unidos
dc.description.fil
Fil: Burguener, German Federico. University of California at Davis; Estados Unidos
dc.description.fil
Fil: Howell, Tyson. University of California at Davis; Estados Unidos
dc.description.fil
Fil: Wang, Hanchao. University Of Haifa; Israel
dc.description.fil
Fil: Fahima, Tzion. University of California at Davis; Estados Unidos
dc.description.fil
Fil: Lukaszewski, Adam. University of California at Davis; Estados Unidos
dc.description.fil
Fil: Moriconi, Jorge Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
dc.description.fil
Fil: Santa Maria, Guillermo Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
dc.description.fil
Fil: Dubcovsky, Jorge. University of California at Davis; Estados Unidos
dc.journal.title
Plant Genome
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://acsess.onlinelibrary.wiley.com/doi/full/10.1002/tpg2.20079
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/tpg2.20079
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