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dc.contributor.author
Lagares, Antonio
dc.contributor.author
Garavaglia, Matías Javier
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Robledo, Natalia Belén
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Valverde, Claudio Fabián
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Goñi, Sandra Elizabeth
dc.contributor.author
Lozano, Mario Enrique
dc.date.available
2022-09-15T14:49:03Z
dc.date.issued
2020-05
dc.identifier.citation
Lagares, Antonio; Garavaglia, Matías Javier; Robledo, Natalia Belén; Valverde, Claudio Fabián; Goñi, Sandra Elizabeth; et al.; Visualising the concept of metabolic regulation in bacteria: a simple laboratory experiment on polyhydroxybutyrate storage; Taylor & Francis; Journal of Biological Education; 56; 1; 5-2020; 95-108
dc.identifier.issn
0021-9266
dc.identifier.uri
http://hdl.handle.net/11336/168936
dc.description.abstract
Regulation is a key concept for understanding the dynamics of metabolism in bacteria. This report outlines a simple laboratory experiment aimed at studying a key form of regulation in bacterial metabolism, exemplifying how microbes switch the flux of carbon towards alternative metabolic fates as a function of nutrient availability and according to their physiological status. The experiment involves the use of a Sinorhizobium meliloti wild-type strain and its isogenic derivative carrying a null mutation in the gene encoding the synthase that generates the storage polymer polyhydroxybutyrate (PHB). These strains serve to characterise the effects of growth phase and carbon availability at the end of balanced growth on the extent of PHB accumulation by rhizobia. The experimental set-up can be completed in a main 4-h laboratory session, followed by an additional 1-h session after an initial incubation for subsequent bacterial-colony counting (48 h), and requires equipment usually available at any university. The protocol described here is performed by the undergraduates of our biochemistry course. We consider this exercise to be a useful complementary tool for spotlighting the concept of metabolic regulation in bacteria and for promoting scientific thinking along with the development of students’ wet-bench laboratory skills.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Taylor & Francis
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
BACTERIA
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METABOLISM
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POLYHYDROXYBUTYRATE
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REGULATION
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UNDERGRADUATE COURSE
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Biología Celular, Microbiología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Visualising the concept of metabolic regulation in bacteria: a simple laboratory experiment on polyhydroxybutyrate storage
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-09-14T14:20:55Z
dc.journal.volume
56
dc.journal.number
1
dc.journal.pagination
95-108
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Lagares, Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Interacciones Biológicas; Argentina
dc.description.fil
Fil: Garavaglia, Matías Javier. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Interacciones Biológicas; Argentina
dc.description.fil
Fil: Robledo, Natalia Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Interacciones Biológicas; Argentina
dc.description.fil
Fil: Valverde, Claudio Fabián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Interacciones Biológicas; Argentina
dc.description.fil
Fil: Goñi, Sandra Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Molecular y Celular; Argentina
dc.description.fil
Fil: Lozano, Mario Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Molecular y Celular; Argentina
dc.journal.title
Journal of Biological Education
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/00219266.2020.1757482
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1080/00219266.2020.1757482
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