Artículo
Expression and purification of recombinant feline interferon in the baculovirus-insect larvae system
Targovnik, Alexandra Marisa
; Villaverde, Marcela Solange
; Arregui, Mariana Bernadett
; Fogar, Mariela A.; Taboga, Oscar Alberto
; Glikin, Gerardo Claudio
; Finocchiaro, Liliana Maria Elena
; Cascone, Osvaldo
; Miranda, Maria Victoria
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Fecha de publicación:
03/2014
Editorial:
Elsevier
Revista:
Process Biochemistry
ISSN:
1359-5113
e-ISSN:
1873-3298
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Feline interferons (FeIFNs) are cytokines with antiviral, antitumor and immunomodulatory functions used as therapeutic agents in a variety of veterinary diseases. In this work, FeIFN-α7 and FeIFN-α7xArg containing eight residues of arginine were expressed in Sf9 cells and insect larvae. At 4 days post-infection (dpi), the concentrations of FeIFN-α7 and FeIFN-α7xArg in suspension culture were (1.28 ± 0.15) × 106 U ml−1 and (1.3 ± 0.2) × 106 U ml−1 respectively. The maximum expression levels of FeIFN-α7 and FeIFN-α7xArg were (3.7 ± 0.2) × 106 U ml−1 and (3.5 ± 0.4) × 106 U ml−1 at 2 dpi in Rachiplusia nu larvae and (1.1 ± 0.2) × 106 U ml−1 and (1.0 ± 0.15) × 106 U ml−1 at 5 dpi in Spodoptera frugiperda larvae respectively. R. nu was a better host for FeIFN-α7 and FeIFN-α7xArg expression. The 8xArg tag did not affect the biological activity of FeIFN-α7 and was useful to promote the FeIFN-α7xArg adsorption on ion exchange chromatography (IEC), allowing its purification in a single step from supernatant culture and R. nu larvae. FeIFN-α7xArg was purified from the larval extract with a yield of 70% and a purification factor of 25 free of viruses. We conclude that R. nu larvae are new low-cost hosts for the expression of recombinant FeIFN-α7.
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Articulos(NANOBIOTEC)
Articulos de INSTITUTO DE NANOBIOTECNOLOGIA
Articulos de INSTITUTO DE NANOBIOTECNOLOGIA
Articulos(OCA HOUSSAY)
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Citación
Targovnik, Alexandra Marisa; Villaverde, Marcela Solange; Arregui, Mariana Bernadett; Fogar, Mariela A.; Taboga, Oscar Alberto; et al.; Expression and purification of recombinant feline interferon in the baculovirus-insect larvae system; Elsevier; Process Biochemistry; 49; 6; 3-2014; 917-926
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