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dc.contributor.author
Urrutia, Pamela J
dc.contributor.author
Bodaleo, Felipe
dc.contributor.author
Bórquez, Daniel A.
dc.contributor.author
Homma, Yuta
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Rozés Salvador, María Victoria
dc.contributor.author
Villablanca, Cristopher
dc.contributor.author
Conde, Cecilia Beatriz
dc.contributor.author
Fukuda, Mitsunori
dc.contributor.author
González Billault, Christian
dc.date.available
2022-09-14T17:27:51Z
dc.date.issued
2021-02
dc.identifier.citation
Urrutia, Pamela J; Bodaleo, Felipe; Bórquez, Daniel A.; Homma, Yuta; Rozés Salvador, María Victoria; et al.; Tuba activates cdc42 during neuronal polarization downstream of the small gtpase rab8a; Society for Neuroscience; Journal of Neuroscience; 41; 8; 2-2021; 1636-1649
dc.identifier.issn
0270-6474
dc.identifier.uri
http://hdl.handle.net/11336/168730
dc.description.abstract
The acquisition of neuronal polarity is a complex molecular process that depends on changes in cytoskeletal dynamics and directed membrane traffic, regulated by the Rho and Rab families of small GTPases, respectively. However, during axon specification, a molecular link that couples these protein families has yet to be identified. In this paper, we describe a new positive feedback loop between Rab8a and Cdc42, coupled by Tuba, a Cdc42-specific guanine nucleotide-exchange factor (GEF), that ensures a single axon generation in rodent hippocampal neurons from embryos of either sex. Accordingly, Rab8a or Tuba gain-of-function generates neurons with supernumerary axons whereas Rab8a or Tuba loss-of-function abrogated axon specification, phenocopying the well-established effect of Cdc42 on neuronal polarity. Although Rab8 and Tuba do not interact physically, the activity of Rab8 is essential to generate a proximal to distal axonal gradient of Tuba in cultured neurons. Tuba-associated and Rab8a-associated polarity defects are also evidenced in vivo, since dominant negative (DN) Rab8a or Tuba knock-down impairs cortical neuronal migration in mice. Our results suggest that Tuba coordinates directed vesicular traffic and cytoskeleton dynamics during neuronal polarization.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Society for Neuroscience
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
AXON SPECIFICATION
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CYTOSKELETON
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NEURONAL DIFFERENTIATION
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NEURONAL POLARITY
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RAB GTPASES
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RHO GTPASES
dc.subject.classification
Neurociencias
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Medicina Básica
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Tuba activates cdc42 during neuronal polarization downstream of the small gtpase rab8a
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-08-30T14:29:55Z
dc.identifier.eissn
1529-2401
dc.journal.volume
41
dc.journal.number
8
dc.journal.pagination
1636-1649
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Washington D.C
dc.description.fil
Fil: Urrutia, Pamela J. Universidad de Chile; Chile. Geroscience Center for Brain Health and Metabolism; Chile
dc.description.fil
Fil: Bodaleo, Felipe. Geroscience Center for Brain Health and Metabolism; Chile. Universidad de Chile; Chile
dc.description.fil
Fil: Bórquez, Daniel A.. Universidad Diego Portales; Chile
dc.description.fil
Fil: Homma, Yuta. Tohoku University; Japón
dc.description.fil
Fil: Rozés Salvador, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
dc.description.fil
Fil: Villablanca, Cristopher. Universidad de Chile; Chile. Geroscience Center for Brain Health and Metabolism; Chile
dc.description.fil
Fil: Conde, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
dc.description.fil
Fil: Fukuda, Mitsunori. Tohoku University; Japón
dc.description.fil
Fil: González Billault, Christian. Universidad de Chile; Chile. Geroscience Center for Brain Health and Metabolism; Chile
dc.journal.title
Journal of Neuroscience
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.jneurosci.org/lookup/doi/10.1523/JNEUROSCI.0633-20.2020
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1523/JNEUROSCI.0633-20.2020
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