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dc.contributor.author
Lovera, Andrea
dc.contributor.author
Belaich, Mariano Nicolas
dc.contributor.author
Villamizar, Laura
dc.contributor.author
Patarroyo, Manuel A.
dc.contributor.author
Barrera, Gloria
dc.date.available
2022-09-13T12:59:42Z
dc.date.issued
2020-05
dc.identifier.citation
Lovera, Andrea; Belaich, Mariano Nicolas; Villamizar, Laura; Patarroyo, Manuel A.; Barrera, Gloria; Enhanced virulence of Beauveria bassiana against Diatraea saccharalis using a soluble recombinant enzyme with endo- and exochitinase activity; Academic Press Inc Elsevier Science; Biological Control; 144; 5-2020; 1-11
dc.identifier.issn
1049-9644
dc.identifier.uri
http://hdl.handle.net/11336/168486
dc.description.abstract
Beauveria bassiana chitinases are involved in degrading the chitin in insects’ exoskeletons and internal structures, and thus are important virulence factors as they participate in initial to final steps of infection. In this work, the B. bassiana (Bv062 isolate) open reading frame (ORF) encoding a chitinase identified as Chit37 (orthologous Bvchit1) was molecularly cloned and expressed in Escherichia coli, and the potential of the recombinant protein (rChit37) to enhance the insecticidal activity of Bv062 conidia against second instar Diatraea saccharalis larvae was studied. rChit37 was produced in both soluble and insoluble fractions of an E. coli culture. Both fractions expressing endo- (90 mU/µL) and exochitinase (170 mU/µL) enzymatic activity, with optimum conditions for enzyme activity of 45 °C and pH 5.0. His-tag affinity chromatography was used to purify the rChit37 from the soluble fraction. Purified rChit37 was then diluted to 200 and 300 µg/mL for use as Bv062 conidia additive (1x106con/mL) in a laboratory bioassay against D. saccharalis larvae. No significant differences were observed between the efficacy of Bv062 conidia applied alone or mixed with 200 µg/mL purified rChit37. However, 300 µg/mL rChit37 increased BV062 conidia insecticidal activity, achieving 96.7% efficacy (14 days post-infection) and 6.2 days median lethal time (LT50), compared to 60% efficacy and 8.9 days for conidia alone. rChit37 addition did not affect conidial viability in terms of germination (96.6% after 24 h) or vigour estimated as germ-tube elongation rate. This work provides proof of concept about soluble recombinant chitinase as an additive to enhance B. bassiana virulence against D. saccharalis.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Academic Press Inc Elsevier Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
BEAUVERIA BASSIANA
dc.subject
CHITINASE
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DIATRAEA SACCHARALIS
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RECOMBINANT PROTEIN
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VIRULENCE
dc.subject.classification
Biología Celular, Microbiología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Enhanced virulence of Beauveria bassiana against Diatraea saccharalis using a soluble recombinant enzyme with endo- and exochitinase activity
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-09-12T14:21:41Z
dc.journal.volume
144
dc.journal.pagination
1-11
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Lovera, Andrea. Universidad Nacional de Colombia; Colombia. Corporación Colombiana de Investigación Agropecuaria; Colombia
dc.description.fil
Fil: Belaich, Mariano Nicolas. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Molecular y Celular. Área Virus de Insectos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Villamizar, Laura. No especifíca;
dc.description.fil
Fil: Patarroyo, Manuel A.. Fundación Instituto de Inmunología; Colombia. Universidad Colegio Mayor de Nuestra Señora del Rosario; Colombia
dc.description.fil
Fil: Barrera, Gloria. Corporación Colombiana de Investigación Agropecuaria; Colombia
dc.journal.title
Biological Control
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1049964419308163
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.biocontrol.2020.104211
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