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dc.contributor.author
Minuti, Andrea
dc.contributor.author
Zhou, Zheng
dc.contributor.author
Graugnard, Daniel E
dc.contributor.author
Rodriguez Zas, Sandra L.
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Palladino, Rafael Alejandro
dc.contributor.author
Cardoso, Felipe C.
dc.contributor.author
Trevisi, Erminio
dc.contributor.author
Loor, Juan J
dc.date.available
2017-05-22T19:57:12Z
dc.date.issued
2015-04
dc.identifier.citation
Minuti, Andrea; Zhou, Zheng; Graugnard, Daniel E; Rodriguez Zas, Sandra L.; Palladino, Rafael Alejandro; et al.; Acute mammary and liver transcriptome responses after an intramammary Escherichia coli lipopolysaccharide challenge in postpartal dairy cows; American Physiological Society; Physiological Reports; 3; 4; 4-2015; 1-12; e12388
dc.identifier.uri
http://hdl.handle.net/11336/16818
dc.description.abstract
The study investigated the effect of an intramammary lipopolysaccharide (LPS) challenge on the bovine mammary and liver transcriptome and its consequences on metabolic biomarkers and liver tissue composition. At 7 days of lactation, 7 cows served as controls (CTR) and 7 cows (LPS) received an intramammary Escherichia coli LPS challenge. The mammary and liver tissues for transcriptomic profiling were biopsied at 2.5 h from challenge. Liver composition was evaluated at 2.5 h and 7 days after challenge, and blood biomarkers were analyzed at 2, 3, 7 and 14 days from challenge. In mammary tissue, the LPS challenge resulted in 189 differentially expressed genes (DEG), with 20 down-regulated and 169 up-regulated. In liver tissue, there were 107 DEG in LPS compared with CTR with 42 down-regulated and 65 up-regulated. In mammary, bioinformatics analysis highlighted that LPS led to activation of NOD-like receptor signaling, Toll-like receptor signaling, RIG-I-like receptor signaling and apoptosis pathways. In liver, LPS resulted in an overall inhibition of fatty acid elongation in mitochondria and activation of the p53 signaling pathway. The LPS challenge induced changes in liver lipid composition, a systemic inflammation (rise of blood ceruloplasmin and bilirubin), and an increase in body fat mobilization. The data suggest that cells within the inflamed mammary gland respond by activating mechanisms of pathogen recognition. However, in the liver the response likely depends on mediators originating from the udder that affect liver functionality and specifically fatty acid metabolism (b-oxidation, ketogenesis, and lipoprotein synthesis).
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Physiological Society
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Mammary
dc.subject
Liver
dc.subject
Lps
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Early Lactation Cows
dc.subject.classification
Producción Animal y Lechería
dc.subject.classification
Producción Animal y Lechería
dc.subject.classification
CIENCIAS AGRÍCOLAS
dc.title
Acute mammary and liver transcriptome responses after an intramammary Escherichia coli lipopolysaccharide challenge in postpartal dairy cows
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-05-18T21:18:37Z
dc.identifier.eissn
2051-817X
dc.journal.volume
3
dc.journal.number
4
dc.journal.pagination
1-12; e12388
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Bethesda
dc.description.fil
Fil: Minuti, Andrea. Universita Cattolica del Sacro Cuore; Italia
dc.description.fil
Fil: Zhou, Zheng. University Of Illinois At Urbana; Estados Unidos
dc.description.fil
Fil: Graugnard, Daniel E. University Of Illinois At Urbana; Estados Unidos
dc.description.fil
Fil: Rodriguez Zas, Sandra L.. University Of Illinois At Urbana; Estados Unidos
dc.description.fil
Fil: Palladino, Rafael Alejandro. Universidad de Buenos Aires. Facultad de Agronomia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Cardoso, Felipe C.. University Of Illinois At Urbana; Estados Unidos
dc.description.fil
Fil: Trevisi, Erminio. Universita Cattolica del Sacro Cuore; Italia
dc.description.fil
Fil: Loor, Juan J. University Of Illinois At Urbana; Estados Unidos
dc.journal.title
Physiological Reports
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.14814/phy2.12388
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://physreports.physiology.org/content/3/4/e12388


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