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dc.contributor.author
Goya, Stephanie
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dc.contributor.author
Galiano, Mónica
dc.contributor.author
Nauwelaers, Inne
dc.contributor.author
Trento, Alfonsina
dc.contributor.author
Openshaw, Peter J.
dc.contributor.author
Mistchenko, Alicia Susana
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dc.contributor.author
Zambon, Maria
dc.contributor.author
Viegas, Mariana
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dc.date.available
2022-09-09T17:55:02Z
dc.date.issued
2020-05
dc.identifier.citation
Goya, Stephanie; Galiano, Mónica; Nauwelaers, Inne; Trento, Alfonsina; Openshaw, Peter J.; et al.; Toward unified molecular surveillance of RSV: A proposal for genotype definition; Wiley Blackwell Publishing, Inc; Influenza And Other Respiratory Viruses; 14; 3; 5-2020; 274-285
dc.identifier.issn
1750-2640
dc.identifier.uri
http://hdl.handle.net/11336/168183
dc.description.abstract
Background: Human respiratory syncytial virus (RSV) is classified into antigenic subgroups A and B. Thirteen genotypes have been defined for RSV-A and 20 for RSV-B, without any consensus on genotype definition. Methods: We evaluated clustering of RSV sequences published in GenBank until February 2018 to define genotypes by using maximum likelihood and Bayesian phylogenetic analyses and average p-distances. Results: We compared the patterns of sequence clustering of complete genomes; the three surface glycoproteins genes (SH, G, and F, single and concatenated); the ectodomain and the 2nd hypervariable region of G gene. Although complete genome analysis achieved the best resolution, the F, G, and G-ectodomain phylogenies showed similar topologies with statistical support comparable to complete genome. Based on the widespread geographic representation and large number of available G-ectodomain sequences, this region was chosen as the minimum region suitable for RSV genotyping. A genotype was defined as a monophyletic cluster of sequences with high statistical support (≥80% bootstrap and ≥0.8 posterior probability), with an intragenotype p-distance ≤0.03 for both subgroups and an intergenotype p-distance ≥0.09 for RSV-A and ≥0.05 for RSV-B. In this work, the number of genotypes was reduced from 13 to three for RSV-A (GA1-GA3) and from 20 to seven for RSV-B (GB1-GB7). Within these, two additional levels of classification were defined: subgenotypes and lineages. Signature amino acid substitutions to complement this classification were also identified. Conclusions: We propose an objective protocol for RSV genotyping suitable for adoption as an international standard to support the global expansion of RSV molecular surveillance.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley Blackwell Publishing, Inc
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
AVERAGE GENETIC DISTANCE
dc.subject
GENOTYPES
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GLOBAL MOLECULAR SURVEILLANCE
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HUMAN ORTHOPNEUMOVIRUS
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HUMAN RESPIRATORY SYNCYTIAL VIRUS
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LINEAGES
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PHYLOGENETIC ANALYSIS
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SUBGENOTYPES
dc.subject.classification
Virología
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Toward unified molecular surveillance of RSV: A proposal for genotype definition
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-09-08T15:19:29Z
dc.identifier.eissn
1750-2659
dc.journal.volume
14
dc.journal.number
3
dc.journal.pagination
274-285
dc.journal.pais
Reino Unido
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dc.journal.ciudad
Londres
dc.description.fil
Fil: Goya, Stephanie. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Laboratorio de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
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Fil: Galiano, Mónica. Public Health England; Reino Unido
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Fil: Nauwelaers, Inne. Imperial College London; Reino Unido
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Fil: Trento, Alfonsina. Instituto de Investigación Hospital 12 de Octubre; España
dc.description.fil
Fil: Openshaw, Peter J.. Imperial College London; Reino Unido
dc.description.fil
Fil: Mistchenko, Alicia Susana. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Laboratorio de Virología; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
dc.description.fil
Fil: Zambon, Maria. Public Health England; Reino Unido
dc.description.fil
Fil: Viegas, Mariana. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Laboratorio de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Influenza And Other Respiratory Viruses
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/irv.12715
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/irv.12715
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