Artículo
Characterization of β-Glucosidase produced by the white rot fungus Flammulina velutipes
Fecha de publicación:
04/09/2014
Editorial:
Korean Society Microbiology
Revista:
Journal Of Microbiology And Biotechnology
ISSN:
1598-2467
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
β-glucosidase production by the white rot fungus Flammulina velutipes CFK 3111 was evaluated using different carbon and nitrogen sources under submerged fermentation. Maximal extracellular enzyme production was 1.6 U/mL corresponding to a culture grown in sucrose 40 g/L and asparagine 10 g/L. High production yield was also obtained with glucose 10 g/L and asparagine 4 g/L medium (0.5 U/mL). Parameters affecting enzyme activity were studied using p-nitro-phenyl-β-D-glucopyranoside as substrate. Optimal activity was found at 50°C and pHs 5.0 to 6.0. Under these conditions, β-glucosidase retained 25% of its initial activity after 12 h of incubation and exhibited a half-life of 5 h. The addition of MgCl2, urea and ethanol enhanced β-glucosidase activity up to 47% while the reactives FeCl2, CuSO4, Cd(NO3)2 and cetyltrimethylammonium bromide inflicted a strong inhibitory effect. Glucose and cellobiose showed also an inhibitory effect on β-glucosidase activity in a concentration dependent manner. The enzyme had an estimated molecular mass of 75 kDa. To the best of our knowledge, F. velutipes CFK 3111 β -glucosidase production is amongst the highest reported up to date, in a basidiomycetous fungus.
Palabras clave:
B-Glucosidase
,
Flammulina Velutipes
,
Submerged Fermentation
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Articulos(INMIBO (EX - PROPLAME))
Articulos de INSTITUTO DE MICOLOGIA Y BOTANICA
Articulos de INSTITUTO DE MICOLOGIA Y BOTANICA
Citación
Mallerman, Julieta; Papinutti, Victor Leandro; Levin, Laura Noemi; Characterization of β-Glucosidase produced by the white rot fungus Flammulina velutipes; Korean Society Microbiology; Journal Of Microbiology And Biotechnology; 25; 1; 4-9-2014; 57-65
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