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dc.contributor.author
Ohtake, Satoshi
dc.contributor.author
Schebor, Carolina Claudia
dc.contributor.author
de Pablo, Juan J.
dc.date.available
2017-05-17T21:49:27Z
dc.date.issued
2006-01
dc.identifier.citation
Ohtake, Satoshi; Schebor, Carolina Claudia; de Pablo, Juan J.; Effects of trehalose on the phase behavior of DPPC–cholesterol unilamellar vesicles; Elsevier Science; Biochimica Et Biophysica Acta - Biomembranes; 1758; 1; 1-2006; 65-73
dc.identifier.issn
0005-2736
dc.identifier.uri
http://hdl.handle.net/11336/16623
dc.description.abstract
A systematic study is presented of the effects of trehalose on the physical properties of extruded DPPC–cholesterol unilamellar vesicles. Particular emphasis is placed on examining how the interactions present in the hydrated state translate into those in the dehydrated state. Observations from HSDSC and DSC are used to examine the phase behavior of hydrated and dehydrated vesicles, respectively. The concentration of trehalose inside and outside the vesicles is manipulated, and is shown to affect the relative stability of the membranes. Our results show for the first time that a combination of high inner and low outer trehalose concentration is able to decrease the gel-to-liquid crystalline phase temperature (Tm), while any other combination will not. Upon dehydration, the Tm of all lipid mixtures increases. The extent of the increase depends on the trehalose distribution across the bilayer. The Tm changes in the same direction with trehalose concentration for both freeze-dried and fully hydrated samples, suggesting that the trehalose distribution across the vesicle membrane, as well as the trehalose–phospholipid interaction, is maintained upon lyophilization. The results presented in this work may aid in the formulation of systems to be used in the lyophilization of liposomes for drug delivery applications.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Trehalose
dc.subject
Membanes
dc.subject
Cholesterol
dc.subject
Dppc
dc.subject.classification
Ingeniería Química
dc.subject.classification
Ingeniería Química
dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS
dc.title
Effects of trehalose on the phase behavior of DPPC–cholesterol unilamellar vesicles
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-05-17T17:47:10Z
dc.journal.volume
1758
dc.journal.number
1
dc.journal.pagination
65-73
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Ohtake, Satoshi. University of Wisconsin; Estados Unidos
dc.description.fil
Fil: Schebor, Carolina Claudia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: de Pablo, Juan J.. University of Wisconsin; Estados Unidos
dc.journal.title
Biochimica Et Biophysica Acta - Biomembranes
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.bbamem.2006.01.002
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0005273606000071
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