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dc.contributor.author
Capriotti, Natalia
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dc.contributor.author
Gioino, Paula
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dc.contributor.author
Ons, Sheila
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dc.contributor.author
Ianowski, Juan Pablo
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dc.date.available
2022-08-11T15:14:15Z
dc.date.issued
2021-04
dc.identifier.citation
Capriotti, Natalia; Gioino, Paula; Ons, Sheila; Ianowski, Juan Pablo; The neuropeptide RhoprCCHamide2 inhibits serotonin-stimulated transcellular Na+ transport across the anterior midgut of the vector of Chagas disease, Rhodnius prolixus; Company of Biologists; Journal of Experimental Biology; 224; 10; 4-2021; 1-30
dc.identifier.issn
0022-0949
dc.identifier.uri
http://hdl.handle.net/11336/165194
dc.description.abstract
Rhodnius prolixus is a blood-feeding insect vector of Trypanosoma cruzi, a protozoan parasite that causes Chagas disease. During each blood meal, the animals ingest large volumes of blood, that may be up to 12 times the unfed body mass. These blood meals impose a significant osmotic stress for the animals due to the hyposmotic condition of the ingested blood compared with the insect’s hemolymph. Thus the insect undergoes a massive postprandial diuresis that allows for the excretion of the plasma fraction of the blood in less than two hours. Diuresis is performed by the excretory system, consisting of the Malpighian tubules and gut, under the control of diuretic and antidiuretic factors. We investigated the ion transport machinery triggered by stimulation with the diuretic factor serotonin in the anterior midgut (i.e. crop) and the effect of the diuretic modulator RhoprCCHamide2. Ussing chamber assays revealed that serotonin-stimulated increase in transepithelial short-circuit current (Isc) was more sensitive to the blockage with amiloride than 5-N-ethyl-N-isopropyl amiloride (EIPA), suggesting the involvement of Na+ channels. Incubation in Na+-free, but not Cl−-free saline, blocked the effect of serotonin on Isc. Moreover, treatment with Na+–K+–2Cl− cotransporter (NKCC) and Na+–Cl− cotransporter (NCC) blockers had no effect on fluid secretion but was blocked by amiloride. Blockage of Na+/K+-ATPase with ouabain inhibited Isc but the H+-ATPase inhibitor bafilomycin had no effect. The neuropeptide RhoprCCHamide2 diminished serotonin-stimulated Isc across the crop. The results suggest that Na+ undergoes active transport via an apical amiloride-sensitive Na+ channel and a basolateral ouabain-sensitive Na+/K+-ATPase, while Cl− is transported through a passive paracellular pathway.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Company of Biologists
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
CCHAMIDE2
dc.subject
CROP
dc.subject
ION TRANSPORT
dc.subject
OSMOREGULATION
dc.subject
RHODNIUS PROLIXUS
dc.subject.classification
Zoología, Ornitología, Entomología, Etología
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
The neuropeptide RhoprCCHamide2 inhibits serotonin-stimulated transcellular Na+ transport across the anterior midgut of the vector of Chagas disease, Rhodnius prolixus
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-08-03T18:13:21Z
dc.journal.volume
224
dc.journal.number
10
dc.journal.pagination
1-30
dc.journal.pais
Reino Unido
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dc.journal.ciudad
Cambridge
dc.description.fil
Fil: Capriotti, Natalia. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
dc.description.fil
Fil: Gioino, Paula. Saskatchewan University; Canadá
dc.description.fil
Fil: Ons, Sheila. Saskatchewan University; Canadá
dc.description.fil
Fil: Ianowski, Juan Pablo. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina
dc.journal.title
Journal of Experimental Biology
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://journals.biologists.com/jeb/article/doi/10.1242/jeb.242272/238763/The-neuropeptide-RhoprCCHamide2-inhibits-serotonin
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1242/jeb.242272
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