Mostrar el registro sencillo del ítem
dc.contributor.author
Khodarahmi, Reza
dc.contributor.author
Akbari, Vali
dc.contributor.author
Mohammadi, Soheila
dc.contributor.author
Farshadnia, Tooraj
dc.contributor.author
Rahimabadi, Mehdi Moradi
dc.contributor.author
Goicoechea, Hector Casimiro
dc.contributor.author
Jalalvand, Ali R.
dc.date.available
2022-08-09T19:21:25Z
dc.date.issued
2021-06
dc.identifier.citation
Khodarahmi, Reza; Akbari, Vali; Mohammadi, Soheila; Farshadnia, Tooraj; Rahimabadi, Mehdi Moradi; et al.; Chemometric modeling of the electrochemical data to investigate proline cis/trans isomeration effect on aggregation of Tau protein; Elsevier; Protein Expression and Purification; 182; 6-2021; 1-14
dc.identifier.issn
1046-5928
dc.identifier.uri
http://hdl.handle.net/11336/164824
dc.description.abstract
Tau protein (Tau) is a proline-rich protein and in this work, we have developed a very interesting strategy based on combination of electrochemistry with chemometric methods to investigate proline cis/trans isomeration effect on the Tau aggregation. To achieve this goal, the proline residues at RTPPK motif have been replaced by alanine to generate RTPAK, RTAPK and RTAAK mutants of the Tau. Then, cyclic voltammetric (CV) responses of the Tau and RTPAK, RTAPK and RTAAK as its mutants in the presence of heparin (HEP) as an anionic inducing agent which could trigger aggregation of the Tau were recorded at physiological conditions every hour during 12 h. Therefore, 48 data sets of titrations were obtained which were handled by chemometric methods to extract useful information about aggregation of the Tau. The data were hard-modeled by EQUISPEC, SQUAD, REACTLAB and SPECFIT to extract useful quantitative information. Our results confirmed that the strength of the binding of the HEP with proteins was obeyed from Tau > RTPAK ~ RTAPK > RTAAK which confirmed that the aggregation of the proteins was obeyed from this order as well. Therefore, aggregation of the Tau is decreased by transforming Cis isomer to Trans even in the presence of an anionic inducing agent such as HEP which may have value for the treatment of Alzheimer's disease.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
AGGREGATION
dc.subject
CHEMOMETRICS
dc.subject
ELECTROCHEMISTRY
dc.subject
HEPARIN
dc.subject
TAU PROTEIN
dc.subject.classification
Química Analítica
dc.subject.classification
Ciencias Químicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Chemometric modeling of the electrochemical data to investigate proline cis/trans isomeration effect on aggregation of Tau protein
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-08-08T15:18:21Z
dc.journal.volume
182
dc.journal.pagination
1-14
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Khodarahmi, Reza. Kermanshah University Of Medical Sciences; Irán
dc.description.fil
Fil: Akbari, Vali. Kermanshah University Of Medical Sciences; Irán
dc.description.fil
Fil: Mohammadi, Soheila. Kermanshah University Of Medical Sciences; Irán
dc.description.fil
Fil: Farshadnia, Tooraj. Kermanshah University Of Medical Sciences; Irán
dc.description.fil
Fil: Rahimabadi, Mehdi Moradi. Kermanshah University Of Medical Sciences; Irán
dc.description.fil
Fil: Goicoechea, Hector Casimiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Analítico y Quimiometría; Argentina
dc.description.fil
Fil: Jalalvand, Ali R.. Kermanshah University Of Medical Sciences; Irán
dc.journal.title
Protein Expression and Purification
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S1046592821000413
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.pep.2021.105858
Archivos asociados