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dc.contributor.author
Ripoll, Magdalena  
dc.contributor.author
Jackson, Erienne  
dc.contributor.author
Trelles, Jorge Abel  
dc.contributor.author
Betancor, Laura  
dc.date.available
2022-08-09T14:14:13Z  
dc.date.issued
2021-11  
dc.identifier.citation
Ripoll, Magdalena; Jackson, Erienne; Trelles, Jorge Abel; Betancor, Laura; Dihydroxyacetone production via heterogeneous biotransformations of crude glycerol; Elsevier Science; Journal of Biotechnology; 340; 11-2021; 102-109  
dc.identifier.issn
0168-1656  
dc.identifier.uri
http://hdl.handle.net/11336/164760  
dc.description.abstract
In this work, several immobilization strategies for Gluconobacter oxydans NBRC 14819 (Gox) were tested in the bioconversion of crude glycerol to dihydroxyacetone (DHA). Agar, agarose and polyacrylamide were evaluated as immobilization matrixes. Glutaraldehyde crosslinked versions of the agar and agarose preparations were also tested. Agar immobilized Gox proved to be the best heterogeneous biocatalyst in the bioconversion of crude glycerol reaching a quantitative production of 50 g/L glycerol into DHA solely in water. Immobilization allowed reutilization for at least eight cycles, reaching four times more DHA than the amount obtained by a single batch of free cells which cannot be reutilized. An increase in scale of 34 times had no impact on DHA productivity. The results obtained herein constitute a contribution to the microbiological production of DHA as they not only attain unprecedented productivities for the reaction with immobilized biocatalysts but also proved that it is feasible to do it in a clean background of solely water that alleviates the cost of downstream processing.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier Science  
dc.rights
info:eu-repo/semantics/restrictedAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/  
dc.subject
BIOTRANSFORMATION  
dc.subject
CRUDE GLYCEROL  
dc.subject
DIHYDROXYACETONE  
dc.subject
GLUCONOBACTER  
dc.subject.classification
Bioprocesamiento Tecnológico, Biocatálisis, Fermentación  
dc.subject.classification
Biotecnología Industrial  
dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS  
dc.title
Dihydroxyacetone production via heterogeneous biotransformations of crude glycerol  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2022-08-02T17:31:12Z  
dc.identifier.eissn
1873-4863  
dc.journal.volume
340  
dc.journal.pagination
102-109  
dc.journal.pais
Países Bajos  
dc.journal.ciudad
Ámsterdam  
dc.description.fil
Fil: Ripoll, Magdalena. Universidad ORT Uruguay; Uruguay. Universidad de la República; Uruguay  
dc.description.fil
Fil: Jackson, Erienne. Universidad ORT Uruguay; Uruguay  
dc.description.fil
Fil: Trelles, Jorge Abel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina  
dc.description.fil
Fil: Betancor, Laura. Universidad ORT Uruguay; Uruguay  
dc.journal.title
Journal of Biotechnology  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0168165621002224  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.jbiotec.2021.08.011