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dc.contributor.author
Ripoll, Magdalena
dc.contributor.author
Jackson, Erienne
dc.contributor.author
Trelles, Jorge Abel
dc.contributor.author
Betancor, Laura
dc.date.available
2022-08-09T14:14:13Z
dc.date.issued
2021-11
dc.identifier.citation
Ripoll, Magdalena; Jackson, Erienne; Trelles, Jorge Abel; Betancor, Laura; Dihydroxyacetone production via heterogeneous biotransformations of crude glycerol; Elsevier Science; Journal of Biotechnology; 340; 11-2021; 102-109
dc.identifier.issn
0168-1656
dc.identifier.uri
http://hdl.handle.net/11336/164760
dc.description.abstract
In this work, several immobilization strategies for Gluconobacter oxydans NBRC 14819 (Gox) were tested in the bioconversion of crude glycerol to dihydroxyacetone (DHA). Agar, agarose and polyacrylamide were evaluated as immobilization matrixes. Glutaraldehyde crosslinked versions of the agar and agarose preparations were also tested. Agar immobilized Gox proved to be the best heterogeneous biocatalyst in the bioconversion of crude glycerol reaching a quantitative production of 50 g/L glycerol into DHA solely in water. Immobilization allowed reutilization for at least eight cycles, reaching four times more DHA than the amount obtained by a single batch of free cells which cannot be reutilized. An increase in scale of 34 times had no impact on DHA productivity. The results obtained herein constitute a contribution to the microbiological production of DHA as they not only attain unprecedented productivities for the reaction with immobilized biocatalysts but also proved that it is feasible to do it in a clean background of solely water that alleviates the cost of downstream processing.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
BIOTRANSFORMATION
dc.subject
CRUDE GLYCEROL
dc.subject
DIHYDROXYACETONE
dc.subject
GLUCONOBACTER
dc.subject.classification
Bioprocesamiento Tecnológico, Biocatálisis, Fermentación
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Biotecnología Industrial
dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS
dc.title
Dihydroxyacetone production via heterogeneous biotransformations of crude glycerol
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-08-02T17:31:12Z
dc.identifier.eissn
1873-4863
dc.journal.volume
340
dc.journal.pagination
102-109
dc.journal.pais
Países Bajos
dc.journal.ciudad
Ámsterdam
dc.description.fil
Fil: Ripoll, Magdalena. Universidad ORT Uruguay; Uruguay. Universidad de la República; Uruguay
dc.description.fil
Fil: Jackson, Erienne. Universidad ORT Uruguay; Uruguay
dc.description.fil
Fil: Trelles, Jorge Abel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina
dc.description.fil
Fil: Betancor, Laura. Universidad ORT Uruguay; Uruguay
dc.journal.title
Journal of Biotechnology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0168165621002224
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.jbiotec.2021.08.011
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