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dc.contributor.author
Taylor, Jennifer A.  
dc.contributor.author
Santiago, Cintia Cecilia  
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Gray, Joe  
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Wodzanowski, Kimberly A.  
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DeMeester, Kristen E.  
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Biboy, Jacob  
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Vollmer, Waldemar  
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Grimes, Catherine L.  
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Salama, Nina R.  
dc.date.available
2022-08-03T14:49:02Z  
dc.date.issued
2021-04  
dc.identifier.citation
Taylor, Jennifer A.; Santiago, Cintia Cecilia; Gray, Joe; Wodzanowski, Kimberly A.; DeMeester, Kristen E.; et al.; Localizing Peptidoglycan Synthesis in Helicobacter pylori using Clickable Metabolic Probes; John Wiley & Sons Inc.; Current Protocols; 1; e80; 4-2021; 1-52  
dc.identifier.issn
1934-2616  
dc.identifier.uri
http://hdl.handle.net/11336/164045  
dc.description.abstract
The bacterial cell wall, composed of peptidoglycan (PG), provides structural integrity for the cell and is responsible for cell shape in most bacteria. Here we present tools to study the cell wall using a clickable PG-specific sugar, 2- alkyne muramic acid (MurNAc-alk), as a metabolic probe. Here we present a new reaction pathway for generating MurNAc-alk. We also include protocols for labeling PG synthesis in Helicobacter pylori, determining the identity of the labeled muropeptides using LC-MS/MS, sample preparation of cells labeled for a short fraction of the doubling time, and visualization using 3D structured illumination microscopy.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
John Wiley & Sons Inc.  
dc.rights
info:eu-repo/semantics/restrictedAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
CELL WALL  
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HELICOBACTER PYLORI  
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LC-MS/MS IDENTIFICATION  
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METABOLIC LABELING  
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MICROSCOPY  
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N-ACETYLMURAMIC ACID  
dc.subject.classification
Biología Celular, Microbiología  
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Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Localizing Peptidoglycan Synthesis in Helicobacter pylori using Clickable Metabolic Probes  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2022-08-02T17:24:19Z  
dc.identifier.eissn
2691-1299  
dc.journal.volume
1  
dc.journal.number
e80  
dc.journal.pagination
1-52  
dc.journal.pais
Estados Unidos  
dc.journal.ciudad
Hoboken, Nueva Jersey, Estados Unidos  
dc.description.fil
Fil: Taylor, Jennifer A.. University of Washington; Estados Unidos. Fred Hutchinson Cancer Research Center; Estados Unidos  
dc.description.fil
Fil: Santiago, Cintia Cecilia. University of Delaware; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Gray, Joe. Newcastle University; Estados Unidos  
dc.description.fil
Fil: Wodzanowski, Kimberly A.. University of Delaware; Estados Unidos  
dc.description.fil
Fil: DeMeester, Kristen E.. University of Delaware; Estados Unidos  
dc.description.fil
Fil: Biboy, Jacob. Newcastle University; Estados Unidos  
dc.description.fil
Fil: Vollmer, Waldemar. Newcastle University; Estados Unidos  
dc.description.fil
Fil: Grimes, Catherine L.. University of Delaware; Estados Unidos  
dc.description.fil
Fil: Salama, Nina R.. University of Washington; Estados Unidos. Fred Hutchinson Cancer Research Center; Estados Unidos  
dc.journal.title
Current Protocols  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/cpz1.80  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://currentprotocols.onlinelibrary.wiley.com/doi/10.1002/cpz1.80  

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