Artículo
One step histological detection and staining of the pten tumor suppressor protein by a single strand dna
Longinotti, María Gloria; Ybarra, Gabriel Omar; Vighi, Susana; Perandones, Claudia; Montserrat, Javier Marcelo
; Yakisich, Juan Sebastian; Grasselli, Mariano
; Radrizzani Helguera, Martin
![Icon](/themes/CONICETDigital/images/conicet.png)
![Icon](/themes/CONICETDigital/images/conicet.png)
![Icon](/themes/CONICETDigital/images/conicet.png)
Fecha de publicación:
01/2021
Editorial:
MDPI AG
Revista:
Diagnostics
ISSN:
2075-4418
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Antibodies are the most used technological tool in histochemistry. However, even with monoclonal antibodies, their standardization is difficult due to variation of biological systems as well as to variability due to the affinity and amplification of the signal arising from secondary peroxidase detection systems. In this article we combined two synthetic molecules to facilitate the standardization of a detection protocol of protein markers in histological sections. The first molecule was an aptamer, a 50-base single-stranded DNA fragment, which recognizes a PTEN tumor suppressor. The second molecule used was also another single stranded 18-base aptamer DNA fragment, which forms a quadruplex structure guanine box. This G-quadruplex recognizes and attaches a molecule of hemin, increasing the catalytic capacity for the hydrogen peroxide. Our results show how the correct structural design of DNA combining an aptamer together with the peroxidase-like DNAzyme allows to detect proteins in histological sections. This tool offers the standardization of the detection of prognostic markers in cancer, in quality and quantity, due to its synthetic nature and its 1:1 antigen:enzyme ratio. This is the first time that reproducible results have been presented in histological sections staining a cancer marker using a single-stranded DNA molecule with dual function.
Palabras clave:
APTAMER
,
DNAZYME-PEROXIDASE
,
ENDOMETRIUM
,
HISTOCHEMISTRY
,
PTEN
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(IMBICE)
Articulos de INST.MULTIDISCIPL.DE BIOLOGIA CELULAR (I)
Articulos de INST.MULTIDISCIPL.DE BIOLOGIA CELULAR (I)
Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Articulos de SEDE CENTRAL
Citación
Longinotti, María Gloria; Ybarra, Gabriel Omar; Vighi, Susana; Perandones, Claudia; Montserrat, Javier Marcelo; et al.; One step histological detection and staining of the pten tumor suppressor protein by a single strand dna; MDPI AG; Diagnostics; 11; 2; 1-2021; 1-13
Compartir
Altmétricas