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Artículo

PsAA9A, a C1-specific AA9 lytic polysaccharide monooxygenase from the white-rot basidiomycete Pycnoporus sanguineus

Garrido, Mercedes María; Landoni, MalenaIcon ; Sabbadin, Federico; Valacco, Maria PiaIcon ; Couto, Alicia SusanaIcon ; Bruce, Neil; Wirth, Sonia AlejandraIcon ; Campos, EleonoraIcon
Fecha de publicación: 09/2020
Editorial: Springer
Revista: Applied Microbiology and Biotechnology
ISSN: 0175-7598
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Bioproductos, Biomateriales, Bioplásticos, Biocombustibles, Bioderivados, etc.; Bioquímica y Biología Molecular

Resumen

Woody biomass represents an important source of carbon on earth, and its global recycling is highly dependent on Agaricomycetes fungi. White-rot Basidiomycetes are a very important group in this regard, as they possess a large and diverse enzymatic repertoire for biomass decomposition. Among these enzymes, the recently discovered lytic polysaccharide monooxygenases (LPMOs) have revolutionized biomass processing with their novel oxidative mechanism of action. The strikingly high representation of LPMOs in fungal genomes raises the question of their functional versatility. In this work, we studied an AA9 LPMO from the white-rot basidiomycete Pycnoporus sanguineus, PsAA9A. Successfully produced as a recombinant secreted protein in Pichia pastoris, PsAA9A was found to be a C1-specific LPMO active on cellulosic substrates, generating native and oxidized cello-oligosaccharides in the presence of an external electron donor. PsAA9A boosted cellulolytic activity of glysoside hydrolases from families GH1, GH5, and GH6.This study serves as a starting point towards understanding the functional versatility and biotechnological potential of this enzymatic family, highly represented in wood decay fungi, in Pycnoporus genus. Key points: • PsAA9A is the first AA9 from P. sanguineus to be characterized. • PsAA9A has activity on cellulose, producing C1-oxidized cello-oligosaccharides. • Boosting activity with GH1, GH5, and GH6 was proven.
Palabras clave: AA9 , CELLULOSE , LPMO , PYCNOPORUS
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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/163629
URL: https://link.springer.com/article/10.1007/s00253-020-10911-6
DOI: http://dx.doi.org/10.1007/s00253-020-10911-6
Colecciones
Articulos (IABIMO)
Articulos de INSTITUTO DE AGROBIOTECNOLOGIA Y BIOLOGIA MOLECULAR
Articulos(CIHIDECAR)
Articulos de CENTRO DE INVESTIGACIONES EN HIDRATOS DE CARBONO
Articulos(IBBEA)
Articulos de INSTITUTO DE BIODIVERSIDAD Y BIOLOGIA EXPERIMENTAL Y APLICADA
Articulos(IQUIBICEN)
Articulos de INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CS. EXACTAS Y NATURALES
Citación
Garrido, Mercedes María; Landoni, Malena; Sabbadin, Federico; Valacco, Maria Pia; Couto, Alicia Susana; et al.; PsAA9A, a C1-specific AA9 lytic polysaccharide monooxygenase from the white-rot basidiomycete Pycnoporus sanguineus; Springer; Applied Microbiology and Biotechnology; 104; 22; 9-2020; 9631-9643
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