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dc.contributor.author
Murphy, Niamh
dc.contributor.author
Macchiaverna, Natalia Paula
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dc.contributor.author
Cardinal, Marta Victoria
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dc.contributor.author
Bhattacharyya, Tapan
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dc.contributor.author
Mertens, Pascal
dc.contributor.author
Zeippen, Nicolas
dc.contributor.author
Gustin, Yves
dc.contributor.author
Gilleman, Quentin
dc.contributor.author
Gurtler, Ricardo Esteban
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dc.contributor.author
Miles, Michael A.
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dc.date.available
2022-07-29T17:00:18Z
dc.date.issued
2019-09
dc.identifier.citation
Murphy, Niamh; Macchiaverna, Natalia Paula; Cardinal, Marta Victoria; Bhattacharyya, Tapan; Mertens, Pascal; et al.; Lineage-specific rapid diagnostic tests can resolve Trypanosoma cruzi TcII/V/VI ecological and epidemiological associations in the Argentine Chaco; BioMed Central; Parasites and Vectors; 12; 1; 9-2019; 1-11
dc.identifier.issn
1756-3305
dc.identifier.uri
http://hdl.handle.net/11336/163561
dc.description.abstract
Background: Trypanosoma cruzi, the protozoan agent of Chagas disease, is comprised of at least 6 genetic lineages (TcI-TcVI). Their geographical distribution, clinical associations and reservoir hosts are not fully elucidated, as genotyping is hampered due to the difficulty in isolating representative populations of organisms. Lineage-specific serological techniques may address these issues. Methods: Trypanosoma cruzi lineage-specific serological assays were performed on human, canine, feline and armadillo sera from the Gran Chaco in northern Argentina, a region of ongoing transmission. Synthetic peptides representing lineage-specific epitopes of the trypomastigote small surface antigen (TSSA) were used in ELISA, and the TcII/V/VI shared epitope peptide (TSSApep-II/V/VI) was used in the Chagas Sero K-SeT rapid diagnostic test (RDT). Results: Chagas Sero K-SeT RDT, using Protein G to detect human and canine IgG, was at least as sensitive as TSSApep-II/V/VI ELISA using specific secondary antibodies. For sera from humans TSSApep-II/V/VI seroprevalence by Chagas Sero K-SeT was 273/393 (69.5%), for dogs 48/73 (65.8%) and for armadillos 1/7 (14.3%); by ELISA for cats 5/19 (26.3%). The seroprevalence for humans was similar to that for Bolivian patients, amongst whom we previously observed an association of TSSApep-II/V/VI seropositivity with severity of cardiomyopathy. In humans, prevalence of TSSApep-II/V/VI recognition was associated with locality, and with increasing and decreasing age within the Qom and Creole populations, respectively. For dogs TSSApep-II/V/VI recognition was associated with being born before community-wide insecticide spraying (P = 0.05) and with Qom household (P < 0.001). Conclusions: We show here that Chagas Sero K-SeT RDT can replace ELISA for TSSApep-II/V/VI serology of humans and dogs; for humans there were statistically significant associations between a positive Chagas Sero K-SeT RDT and being resident in Area IV, and for dogs association with Qom household or with being born before the mass spraying campaign; we also show that with cats the TcII/V/VI epitope can be detected by ELISA. We assessed the lineage distribution in an unprecedented 83% of the human T. cruzi-seropositive population. These results form the basis for more detailed studies, enabling rapid in-the-field surveillance of the distribution and clustering of these lineages among humans and mammalian reservoirs of T. cruzi infection.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
BioMed Central
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
ARGENTINA
dc.subject
CHAGAS DISEASE
dc.subject
ELISA
dc.subject
LINEAGE-SPECIFIC
dc.subject
RAPID DIAGNOSTIC TEST
dc.subject
SEROLOGY
dc.subject
TRYPANOSOMA CRUZI
dc.subject.classification
Epidemiología
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dc.subject.classification
Ciencias de la Salud
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dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
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dc.title
Lineage-specific rapid diagnostic tests can resolve Trypanosoma cruzi TcII/V/VI ecological and epidemiological associations in the Argentine Chaco
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-11-20T14:47:06Z
dc.journal.volume
12
dc.journal.number
1
dc.journal.pagination
1-11
dc.journal.pais
Reino Unido
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dc.journal.ciudad
Londres
dc.description.fil
Fil: Murphy, Niamh. London School of Hygiene & Tropical Medicine; Reino Unido
dc.description.fil
Fil: Macchiaverna, Natalia Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina
dc.description.fil
Fil: Cardinal, Marta Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina
dc.description.fil
Fil: Bhattacharyya, Tapan. London School of Hygiene & Tropical Medicine; Reino Unido
dc.description.fil
Fil: Mertens, Pascal. No especifíca;
dc.description.fil
Fil: Zeippen, Nicolas. No especifíca;
dc.description.fil
Fil: Gustin, Yves. No especifíca;
dc.description.fil
Fil: Gilleman, Quentin. No especifíca;
dc.description.fil
Fil: Gurtler, Ricardo Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina
dc.description.fil
Fil: Miles, Michael A.. London School Of Hygiene & Tropical Medicine; Reino Unido
dc.journal.title
Parasites and Vectors
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-019-3681-7
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1186/s13071-019-3681-7
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