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dc.contributor.author
Didierlaurent, Arnaud
dc.contributor.author
Ramirez, Juan-Carlos
dc.contributor.author
Gherardi, Maria Magdalena
dc.contributor.author
Zimmerli, Simone C.
dc.contributor.author
Graf, Marcus
dc.contributor.author
Orbea, Hans Acha
dc.contributor.author
Pantaleo, Giuseppe
dc.contributor.author
Wagner, Ralf
dc.contributor.author
Esteban, Mariano
dc.contributor.author
Kraehenbuhl, Jean Pierre
dc.contributor.author
Sirard, Jean Claude
dc.date.available
2022-07-22T11:05:19Z
dc.date.issued
2004-09
dc.identifier.citation
Didierlaurent, Arnaud; Ramirez, Juan-Carlos; Gherardi, Maria Magdalena; Zimmerli, Simone C.; Graf, Marcus; et al.; Attenuated poxviruses expressing a synthetic HIV protein stimulate HLA-A2-restricted cytotoxic T-cell responses; Elsevier; Vaccine; 22; 25-26; 9-2004; 3395-3403
dc.identifier.issn
0264-410X
dc.identifier.uri
http://hdl.handle.net/11336/162854
dc.description.abstract
Efficient HIV vaccines have to trigger cell-mediated immunity directed against various viral antigens. However little is known about the breadth of the response induced by vaccines carrying multiple proteins. Here, we report on the immunogenicity of a construct harbouring a fusion of the HIV-1 IIIB gag, pol and nef genes (gpn) designed for optimal safety and equimolar expression of the HIV proteins. The attenuated poxviruses, MVA and NYVAC, harbouring the gpn construct, induced potent immune responses in conventional mice characterised by stimulation of Gpn-specific IFN-γ-producing cells and cytotoxic T cells. In HLA-A2 transgenic mice, recombinant MVA elicited cytotoxic responses against epitopes recognised in most HLA-A2+ HIV-1-infected individuals. We also found that the MVA vaccine triggered the in vitro expansion of peripheral blood cells isolated from a HIV-1-seropositive patient and with similar specificity as found in immunised HLA-A2 transgenic mice. In conclusion, the synthetic HIV polyantigen Gpn delivered by MVA is immunogenic, efficiently processed and presented by human MHC class I molecules.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
HIV VACCINE
dc.subject
HLA-A2
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RECOMBINANT POXVIRUSES
dc.subject.classification
Inmunología
dc.subject.classification
Medicina Básica
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Attenuated poxviruses expressing a synthetic HIV protein stimulate HLA-A2-restricted cytotoxic T-cell responses
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-07-15T15:24:14Z
dc.journal.volume
22
dc.journal.number
25-26
dc.journal.pagination
3395-3403
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Didierlaurent, Arnaud. Universite de Lausanne; Suiza
dc.description.fil
Fil: Ramirez, Juan-Carlos. Consejo Superior de Investigaciones Científicas; España
dc.description.fil
Fil: Gherardi, Maria Magdalena. Consejo Superior de Investigaciones Científicas; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Zimmerli, Simone C.. No especifíca;
dc.description.fil
Fil: Graf, Marcus. No especifíca;
dc.description.fil
Fil: Orbea, Hans Acha. Universite de Lausanne; Suiza
dc.description.fil
Fil: Pantaleo, Giuseppe. No especifíca;
dc.description.fil
Fil: Wagner, Ralf. No especifíca;
dc.description.fil
Fil: Esteban, Mariano. Consejo Superior de Investigaciones Científicas; España
dc.description.fil
Fil: Kraehenbuhl, Jean Pierre. Universite de Lausanne; Suiza
dc.description.fil
Fil: Sirard, Jean Claude. Universite de Lausanne; Suiza
dc.journal.title
Vaccine
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0264410X04001872
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.vaccine.2004.02.025
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