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dc.contributor.author
Didierlaurent, Arnaud  
dc.contributor.author
Ramirez, Juan-Carlos  
dc.contributor.author
Gherardi, Maria Magdalena  
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Zimmerli, Simone C.  
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Graf, Marcus  
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Orbea, Hans Acha  
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Pantaleo, Giuseppe  
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Wagner, Ralf  
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Esteban, Mariano  
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Kraehenbuhl, Jean Pierre  
dc.contributor.author
Sirard, Jean Claude  
dc.date.available
2022-07-22T11:05:19Z  
dc.date.issued
2004-09  
dc.identifier.citation
Didierlaurent, Arnaud; Ramirez, Juan-Carlos; Gherardi, Maria Magdalena; Zimmerli, Simone C.; Graf, Marcus; et al.; Attenuated poxviruses expressing a synthetic HIV protein stimulate HLA-A2-restricted cytotoxic T-cell responses; Elsevier; Vaccine; 22; 25-26; 9-2004; 3395-3403  
dc.identifier.issn
0264-410X  
dc.identifier.uri
http://hdl.handle.net/11336/162854  
dc.description.abstract
Efficient HIV vaccines have to trigger cell-mediated immunity directed against various viral antigens. However little is known about the breadth of the response induced by vaccines carrying multiple proteins. Here, we report on the immunogenicity of a construct harbouring a fusion of the HIV-1 IIIB gag, pol and nef genes (gpn) designed for optimal safety and equimolar expression of the HIV proteins. The attenuated poxviruses, MVA and NYVAC, harbouring the gpn construct, induced potent immune responses in conventional mice characterised by stimulation of Gpn-specific IFN-γ-producing cells and cytotoxic T cells. In HLA-A2 transgenic mice, recombinant MVA elicited cytotoxic responses against epitopes recognised in most HLA-A2+ HIV-1-infected individuals. We also found that the MVA vaccine triggered the in vitro expansion of peripheral blood cells isolated from a HIV-1-seropositive patient and with similar specificity as found in immunised HLA-A2 transgenic mice. In conclusion, the synthetic HIV polyantigen Gpn delivered by MVA is immunogenic, efficiently processed and presented by human MHC class I molecules.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
HIV VACCINE  
dc.subject
HLA-A2  
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RECOMBINANT POXVIRUSES  
dc.subject.classification
Inmunología  
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Medicina Básica  
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CIENCIAS MÉDICAS Y DE LA SALUD  
dc.title
Attenuated poxviruses expressing a synthetic HIV protein stimulate HLA-A2-restricted cytotoxic T-cell responses  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2022-07-15T15:24:14Z  
dc.journal.volume
22  
dc.journal.number
25-26  
dc.journal.pagination
3395-3403  
dc.journal.pais
Países Bajos  
dc.journal.ciudad
Amsterdam  
dc.description.fil
Fil: Didierlaurent, Arnaud. Universite de Lausanne; Suiza  
dc.description.fil
Fil: Ramirez, Juan-Carlos. Consejo Superior de Investigaciones Científicas; España  
dc.description.fil
Fil: Gherardi, Maria Magdalena. Consejo Superior de Investigaciones Científicas; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Zimmerli, Simone C.. No especifíca;  
dc.description.fil
Fil: Graf, Marcus. No especifíca;  
dc.description.fil
Fil: Orbea, Hans Acha. Universite de Lausanne; Suiza  
dc.description.fil
Fil: Pantaleo, Giuseppe. No especifíca;  
dc.description.fil
Fil: Wagner, Ralf. No especifíca;  
dc.description.fil
Fil: Esteban, Mariano. Consejo Superior de Investigaciones Científicas; España  
dc.description.fil
Fil: Kraehenbuhl, Jean Pierre. Universite de Lausanne; Suiza  
dc.description.fil
Fil: Sirard, Jean Claude. Universite de Lausanne; Suiza  
dc.journal.title
Vaccine  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0264410X04001872  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.vaccine.2004.02.025