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Artículo

Nitric Oxide and Superoxide Anion Production During Heparin-Induced Capacitation in Cryopreserved Bovine Spermatozoa

Rodriguez, P. C.; Valdez, Laura BatrizIcon ; Zaobornyj, TamaraIcon ; Boveris, Alberto AntonioIcon ; Beconi, Martha Teresa
Fecha de publicación: 02/2011
Editorial: Wiley Blackwell Publishing, Inc
Revista: Reproduction in Domestic Animals
ISSN: 0936-6768
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Veterinarias

Resumen

The aim of this work was to quantify NO, O2− and ONOO− production during heparin-induced capacitation of cryopreserved bovine spermatozoa. A time dependent hyperbolic increase was observed for heparin-dependent capacitation, O2 uptake, and NO production. Conversely, O2− production was increased during the first 15 min of incubation, showing a decrease from this time until 45 min. At 15 min of heparin incubation, a threefold increase in O2 consumption (5.9 ± 0.6 nmol/min × 107 cells), an enhancement in NO release (1.1 ± 0.2 nmol/min × 107 cells), and a five-fold increase in O2− production (1.3 ± 0.07 nmol/min × 107 cells), were observed. Peroxynitrite production rate was estimated taking into account NO and O2− generation and the second-order rate constant of the reaction between these species. To conclude, heparin-induced capacitation of cryopreserved bovine spermatozoa activates (i) mitochondrial O2 uptake by high ADP levels due to increased energy requirements, (ii) NO production by a constitutive NOS and (iii) O2− production by a membrane-bound NAD(P)H oxidase. The products of both enzymes are released to the extracellular space and could be involved in the process of sperm capacitation.
Palabras clave: Nitric Oxide Production , Superoxide Anion Production , Bovine Spermatozoa , Heparin-Induced Capacitation
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/16284
URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0531.2010.01583.x/abstract
DOI: http://dx.doi.org/10.1111/j.1439-0531.2010.01583.x
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Articulos(OCA HOUSSAY)
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Citación
Rodriguez, P. C.; Valdez, Laura Batriz; Zaobornyj, Tamara; Boveris, Alberto Antonio; Beconi, Martha Teresa; Nitric Oxide and Superoxide Anion Production During Heparin-Induced Capacitation in Cryopreserved Bovine Spermatozoa; Wiley Blackwell Publishing, Inc; Reproduction in Domestic Animals; 46; 1; 2-2011; 74-81
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