Artículo
Chemical modifications of globular proteins phototriggered by an endogenous photosensitizer
Reid, Lara Olivia
; Dantola, Maria Laura
; Petroselli, Gabriela
; Erra Balsells, Rosa
; Miranda, Miguel A.; Lhiaubet Vallet, Virginie; Thomas, Andrés Héctor
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Fecha de publicación:
10/2019
Editorial:
American Chemical Society
Revista:
Chemical Research In Toxicology (Washington)
ISSN:
0893-228X
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The main goal of the present work was to investigate the damages photoinduced by pterin (Ptr), an endogenous photosensitizer present in human skin under pathological conditions, on a globular protein such as ubiquitin (Ub). Particular attention has been paid on the formation of covalent adducts between Ptr and the protein that can behave as photoantigen and provoke an immune system response. Here, a multifaceted approach including UV-visible spectrophotometry, fluorescence spectroscopy, electrophoresis, size exclusion chromatography, and mass spectrometry is used to establish the Ub changes triggered by UV-A irradiation in the presence of Ptr. Under anaerobic conditions, the only reaction corresponds to the formation of a covalently bound Ptr-Ub adduct that retains the spectroscopic properties of the free photosensitizer. A more complex scheme is observed in air-equilibrated solutions with the occurrence of three different processes, that is, formation of a Ptr-Ub adduct, dimerization, and fragmentation of the protein.
Palabras clave:
PHOTOSENSITIZER
,
UBIQUITIN
,
PTERIN
,
MASS SPECTROMETRY
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Licencia
Identificadores
Colecciones
Articulos(CIHIDECAR)
Articulos de CENTRO DE INVESTIGACIONES EN HIDRATOS DE CARBONO
Articulos de CENTRO DE INVESTIGACIONES EN HIDRATOS DE CARBONO
Citación
Reid, Lara Olivia; Dantola, Maria Laura; Petroselli, Gabriela; Erra Balsells, Rosa; Miranda, Miguel A.; et al.; Chemical modifications of globular proteins phototriggered by an endogenous photosensitizer; American Chemical Society; Chemical Research In Toxicology (Washington); 32; 11; 10-2019; 2250-2259
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