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dc.contributor.author
Montesino, M.
dc.contributor.author
Sala, Adriana Andrea
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dc.contributor.author
Crespillo, M.
dc.contributor.author
Albarrán, C.
dc.contributor.author
Alonso, A.
dc.contributor.author
Álvarez Iglesias, V.
dc.contributor.author
Cano, J. A.
dc.contributor.author
Carvalho, M.
dc.contributor.author
Corach, Daniel
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dc.contributor.author
Cruz, C.
dc.contributor.author
Di Lonardo, A.
dc.contributor.author
Espinheira, R.
dc.contributor.author
Farfán, M. J.
dc.contributor.author
Filippini, S.
dc.contributor.author
García Hirschfeld, J.
dc.contributor.author
Hernández, A.
dc.contributor.author
Lima, G.
dc.contributor.author
López Cubría, C. M.
dc.contributor.author
López Soto, M.
dc.contributor.author
Pagano, S.
dc.contributor.author
Paredes, M.
dc.contributor.author
Pinheiro, M. F.
dc.contributor.author
RodríguezMonge, A. M.
dc.contributor.author
Sala, A.
dc.contributor.author
Sóñora, S.
dc.contributor.author
Sumita, D. R.
dc.contributor.author
Vide, M. C.
dc.contributor.author
Whittle, M. R.
dc.contributor.author
Zurita, A.
dc.contributor.author
Prieto, L.
dc.date.available
2022-07-19T10:27:56Z
dc.date.issued
2007-05
dc.identifier.citation
Montesino, M.; Sala, Adriana Andrea; Crespillo, M.; Albarrán, C.; Alonso, A.; et al.; Analysis of body fluid mixtures by mtDNA sequencing: An inter-laboratory study of the GEP-ISFG working group; Elsevier Ireland; Forensic Science International; 168; 1; 5-2007; 42-56
dc.identifier.issn
0379-0738
dc.identifier.uri
http://hdl.handle.net/11336/162437
dc.description.abstract
The mitochondrial DNA (mtDNA) working group of the GEP-ISFG (Spanish and Portuguese Group of the International Society for Forensic Genetics) carried out an inter-laboratory exercise consisting of the analysis of mtDNA sequencing patterns in mixed stains (saliva/semen and blood/semen). Mixtures were prepared with saliva or blood from a female donor and three different semen dilutions (pure, 1:10 and 1:20) in order to simulate forensic casework. All labs extracted the DNA by preferential lysis and amplified and sequenced the first mtDNA hypervariable region (HVS-I). Autosomal and Y-STR markers were also analysed in order to compare nuclear and mitochondrial results from the same DNA extracts. A mixed stain prepared using semen from a vasectomized individual was also analysed. The results were reasonably consistent among labs for the first fractions but not for the second ones, for which some laboratories reported contamination problems. In the first fractions, both the female and male haplotypes were generally detected in those samples prepared with undiluted semen. In contrast, most of the mixtures prepared with diluted semen only yielded the female haplotype, suggesting that the mtDNA copy number per cell is smaller in semen than in saliva or blood. Although the detection level of the male component decreased in accordance with the degree of semen dilution, it was found that the loss of signal was not consistently uniform throughout each electropherogram. Moreover, differences between mixtures prepared from different donors and different body fluids were also observed. We conclude that the particular characteristics of each mixed stain can deeply influence the interpretation of the mtDNA evidence in forensic mixtures (leading in some cases to false exclusions). In this sense, the implementation of preliminary tests with the aim of identifying the fluids involved in the mixture is an essential tool. In addition, in order to prevent incorrect conclusions in the interpretation of electropherograms we strongly recommend: (i) the use of additional sequencing primers to confirm the sequencing results and (ii) interpreting the results to the light of the phylogenetic perspective.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Ireland
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
HAPLOTYPE
dc.subject
HVS-I
dc.subject
MTDNA MIXTURE
dc.subject
MTDNA PHYLOGENY
dc.subject
PREFERENTIAL LYSIS
dc.subject
SEQUENCING
dc.subject.classification
Otras Ciencias Biológicas
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Analysis of body fluid mixtures by mtDNA sequencing: An inter-laboratory study of the GEP-ISFG working group
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-07-15T15:22:52Z
dc.journal.volume
168
dc.journal.number
1
dc.journal.pagination
42-56
dc.journal.pais
Irlanda
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dc.description.fil
Fil: Montesino, M.. No especifíca;
dc.description.fil
Fil: Sala, Adriana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Crespillo, M.. Instituto Nacional de Toxicologia y Ciencias Forenses; España
dc.description.fil
Fil: Albarrán, C.. Instituto Nacional de Toxicologia y Ciencias Forenses; España
dc.description.fil
Fil: Alonso, A.. Universidad de Santiago de Compostela; España
dc.description.fil
Fil: Álvarez Iglesias, V.. No especifíca;
dc.description.fil
Fil: Cano, J. A.. No especifíca;
dc.description.fil
Fil: Carvalho, M.. No especifíca;
dc.description.fil
Fil: Corach, Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Cruz, C.. No especifíca;
dc.description.fil
Fil: Di Lonardo, A.. No especifíca;
dc.description.fil
Fil: Espinheira, R.. No especifíca;
dc.description.fil
Fil: Farfán, M. J.. No especifíca;
dc.description.fil
Fil: Filippini, S.. No especifíca;
dc.description.fil
Fil: García Hirschfeld, J.. No especifíca;
dc.description.fil
Fil: Hernández, A.. No especifíca;
dc.description.fil
Fil: Lima, G.. No especifíca;
dc.description.fil
Fil: López Cubría, C. M.. No especifíca;
dc.description.fil
Fil: López Soto, M.. No especifíca;
dc.description.fil
Fil: Pagano, S.. No especifíca;
dc.description.fil
Fil: Paredes, M.. No especifíca;
dc.description.fil
Fil: Pinheiro, M. F.. No especifíca;
dc.description.fil
Fil: RodríguezMonge, A. M.. No especifíca;
dc.description.fil
Fil: Sala, A.. No especifíca;
dc.description.fil
Fil: Sóñora, S.. No especifíca;
dc.description.fil
Fil: Sumita, D. R.. No especifíca;
dc.description.fil
Fil: Vide, M. C.. No especifíca;
dc.description.fil
Fil: Whittle, M. R.. No especifíca;
dc.description.fil
Fil: Zurita, A.. No especifíca;
dc.description.fil
Fil: Prieto, L.. No especifíca;
dc.journal.title
Forensic Science International
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S037907380600449X
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.forsciint.2006.06.066
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