Show simple item record

dc.contributor.author Diaz, M.
dc.contributor.author Garbus, Ingrid
dc.contributor.author Stamova, B.
dc.contributor.author Echenique, Carmen Viviana
dc.date.available 2017-05-09T18:36:14Z
dc.date.issued 2011-11-30
dc.identifier.citation Diaz, M.; Garbus, Ingrid; Stamova, B.; Echenique, Carmen Viviana; Functional characterization of three cDNA libraries from the diploid wheat Triticum monoccocum (AmAm) with different growth habits; Akademiai Kiado Rt; Cereal Research Communications; 39; 4; 30-11-2011; 475-486
dc.identifier.issn 0133-3720
dc.identifier.uri http://hdl.handle.net/11336/16150
dc.description.abstract The diploid wheat Triticum monococcum (2n = 2x = 14, A mA m) constitutes an excellent and simplified model to study wheat genome expression since it has lower genome complexity compared to polyploid wheat species.The analysis of a collection of near 10,000 ESTs obtained from two libraries of T. monococcum (TM011XXX and TM043E1X) and one from T. monococcum ssp. aegilopoides (TM046E1X), lead to 2246, 1843 and 2405 unigenes, respectively. The unigenes from each library were individually analyzed through the Blast2Go interface revealing higher identity compared to EST databases. However, a low percentage of sequences showed significant similarity with Triticum databases, reflecting that several novel Triticum expressed genes have been identified in these libraries. The sequences annotation and classification under GO categories showed similar distribution for the three libraries, but differences were identified in some subcategories.When a virtual unique library was constructed including the sequences from the three libraries, seventeen genes were found to be over-expressed between reproductive and vegetative tissues and thirty-five showed genotype-specific expression. These differentially expressed genes included several regulatory proteins and some unreported sequences. Some of these differentially expressed genes were validated by qRT-PCR. The exploration for specific polymorphisms allowed the identification of several SNPs.These EST libraries constitute a valuable tool for the assessment of active metabolic pathways in vegetative and reproductive tissues in diploid wheat species. A more detailed assessment of expression profiles of genotypes with spring and winter growth habits would allow the identification of the transcriptomic strategies developed under the pressure of these antagonistic growth conditions.
dc.format application/pdf
dc.language.iso eng
dc.publisher Akademiai Kiado Rt
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject DIPLOID WHEAT
dc.subject CDNA LIBRARIES
dc.subject ESTS
dc.subject DIGITAL DIFFERENTIAL EXPRESSION
dc.subject QRT-PCR
dc.subject.classification Otras Biotecnología Agropecuaria
dc.subject.classification Biotecnología Agropecuaria
dc.subject.classification CIENCIAS AGRÍCOLAS
dc.title Functional characterization of three cDNA libraries from the diploid wheat Triticum monoccocum (AmAm) with different growth habits
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2017-05-02T18:58:29Z
dc.identifier.eissn 1788-9170
dc.journal.volume 39
dc.journal.number 4
dc.journal.pagination 475-486
dc.journal.pais Hungría
dc.journal.ciudad Szeged
dc.description.fil Fil: Diaz, M.. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
dc.description.fil Fil: Garbus, Ingrid. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; Argentina
dc.description.fil Fil: Stamova, B.. University Of California; Estados Unidos
dc.description.fil Fil: Echenique, Carmen Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; Argentina
dc.journal.title Cereal Research Communications
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://akademiai.com/doi/abs/10.1556/CRC.39.2011.4.2
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1556/CRC.39.2011.4.2


Archivos asociados

Icon
Blocked Acceso no disponible

This item appears in the following Collection(s)

Show simple item record

info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)