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dc.contributor.author
Diaz, M.
dc.contributor.author
Garbus, Ingrid
dc.contributor.author
Stamova, B.
dc.contributor.author
Echenique, Carmen Viviana
dc.date.available
2017-05-09T18:36:14Z
dc.date.issued
2011-11-30
dc.identifier.citation
Diaz, M.; Garbus, Ingrid; Stamova, B.; Echenique, Carmen Viviana; Functional characterization of three cDNA libraries from the diploid wheat Triticum monoccocum (AmAm) with different growth habits; Akademiai Kiado Rt; Cereal Research Communications; 39; 4; 30-11-2011; 475-486
dc.identifier.issn
0133-3720
dc.identifier.uri
http://hdl.handle.net/11336/16150
dc.description.abstract
The diploid wheat Triticum monococcum (2n = 2x = 14, A mA m) constitutes an excellent and simplified model to study wheat genome expression since it has lower genome complexity compared to polyploid wheat species.The analysis of a collection of near 10,000 ESTs obtained from two libraries of T. monococcum (TM011XXX and TM043E1X) and one from T. monococcum ssp. aegilopoides (TM046E1X), lead to 2246, 1843 and 2405 unigenes, respectively. The unigenes from each library were individually analyzed through the Blast2Go interface revealing higher identity compared to EST databases. However, a low percentage of sequences showed significant similarity with Triticum databases, reflecting that several novel Triticum expressed genes have been identified in these libraries. The sequences annotation and classification under GO categories showed similar distribution for the three libraries, but differences were identified in some subcategories.When a virtual unique library was constructed including the sequences from the three libraries, seventeen genes were found to be over-expressed between reproductive and vegetative tissues and thirty-five showed genotype-specific expression. These differentially expressed genes included several regulatory proteins and some unreported sequences. Some of these differentially expressed genes were validated by qRT-PCR. The exploration for specific polymorphisms allowed the identification of several SNPs.These EST libraries constitute a valuable tool for the assessment of active metabolic pathways in vegetative and reproductive tissues in diploid wheat species. A more detailed assessment of expression profiles of genotypes with spring and winter growth habits would allow the identification of the transcriptomic strategies developed under the pressure of these antagonistic growth conditions.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Akademiai Kiado Rt
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Diploid Wheat
dc.subject
Cdna Libraries
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Ests
dc.subject
Digital Differential Expression
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Qrt-Pcr
dc.subject.classification
Otras Biotecnología Agropecuaria
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Biotecnología Agropecuaria
dc.subject.classification
CIENCIAS AGRÍCOLAS
dc.title
Functional characterization of three cDNA libraries from the diploid wheat Triticum monoccocum (AmAm) with different growth habits
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-05-02T18:58:29Z
dc.identifier.eissn
1788-9170
dc.journal.volume
39
dc.journal.number
4
dc.journal.pagination
475-486
dc.journal.pais
Hungría
dc.journal.ciudad
Szeged
dc.description.fil
Fil: Diaz, M.. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
dc.description.fil
Fil: Garbus, Ingrid. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; Argentina
dc.description.fil
Fil: Stamova, B.. University Of California; Estados Unidos
dc.description.fil
Fil: Echenique, Carmen Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; Argentina
dc.journal.title
Cereal Research Communications
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://akademiai.com/doi/abs/10.1556/CRC.39.2011.4.2
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1556/CRC.39.2011.4.2


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