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dc.contributor.author
Estrada Navarrete, Georgina
dc.contributor.author
Cruz Mireles, Neftaly
dc.contributor.author
Lascano, Hernan Ramiro
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dc.contributor.author
Alvarado Affantranger, Xóchitl
dc.contributor.author
Hernández Barrera, Alejandra
dc.contributor.author
Barraza, Aarón
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Olivares, Juan E.
dc.contributor.author
Arthikala, Manoj Kumar
dc.contributor.author
Cárdenas, Luis
dc.contributor.author
Quinto, Carmen
dc.contributor.author
Sanchez, Federico
dc.date.available
2022-07-05T10:29:19Z
dc.date.issued
2016-09
dc.identifier.citation
Estrada Navarrete, Georgina; Cruz Mireles, Neftaly; Lascano, Hernan Ramiro; Alvarado Affantranger, Xóchitl; Hernández Barrera, Alejandra; et al.; An autophagy-related kinase is essential for the symbiotic relationship between phaseolus vulgaris and both rhizobia and arbuscular mycorrhizal fungi; American Society of Plant Biologist; Plant Cell; 28; 9; 9-2016; 2326-2341
dc.identifier.issn
1040-4651
dc.identifier.uri
http://hdl.handle.net/11336/161260
dc.description.abstract
Eukaryotes contain three types of lipid kinases that belong to the phosphatidylinositol 3-kinase (PI3K) family. In plants and Saccharomyces cerevisiae, only PI3K class III family members have been identified. These enzymes regulate the innate immune response, intracellular trafficking, autophagy, and senescence. Here, we report that RNAi-mediated downregulation of common bean (Phaseolus vulgaris) PI3K severely impaired symbiosis in composite P. vulgaris plants with endosymbionts such as Rhizobium tropici and Rhizophagus irregularis. Downregulation of Pv-PI3K was associated with a marked decrease in root hair growth and curling. Additionally, infection thread growth, root-nodule number, and symbiosome formation in root nodule cells were severely affected. Interestingly, root colonization by AM fungi and the formation of arbuscules were also abolished in PI3K loss-of-function plants. Furthermore, the transcript accumulation of genes encoding proteins known to interact with PI3K to form protein complexes involved in autophagy was drastically reduced in these transgenic roots. RNAimediated downregulation of one of these genes, Beclin1/Atg6, resulted in a similar phenotype as observed for transgenic roots in which Pv-PI3K had been downregulated. Our findings show that an autophagy-related process is crucial for the mutualistic interactions of P. vulgaris with beneficial microorganisms.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Society of Plant Biologist
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Gene silencing - RNA interference/RNAi,
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Plant-microbe symbiosis
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Autophagy
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Root development - root nodules
dc.subject.classification
Bioquímica y Biología Molecular
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
An autophagy-related kinase is essential for the symbiotic relationship between phaseolus vulgaris and both rhizobia and arbuscular mycorrhizal fungi
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-06-21T18:37:15Z
dc.identifier.eissn
1532-298X
dc.journal.volume
28
dc.journal.number
9
dc.journal.pagination
2326-2341
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
Rockville
dc.description.fil
Fil: Estrada Navarrete, Georgina. Universidad Nacional Autónoma de México; México
dc.description.fil
Fil: Cruz Mireles, Neftaly. Universidad Nacional Autónoma de México; México
dc.description.fil
Fil: Lascano, Hernan Ramiro. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
dc.description.fil
Fil: Alvarado Affantranger, Xóchitl. Universidad Nacional Autónoma de México; México
dc.description.fil
Fil: Hernández Barrera, Alejandra. Universidad Nacional Autónoma de México; México
dc.description.fil
Fil: Barraza, Aarón. Universidad Nacional Autónoma de México; México
dc.description.fil
Fil: Olivares, Juan E.. Universidad Nacional Autónoma de México; México
dc.description.fil
Fil: Arthikala, Manoj Kumar. Universidad Nacional Autónoma de México; México
dc.description.fil
Fil: Cárdenas, Luis. Universidad Nacional Autónoma de México; México
dc.description.fil
Fil: Quinto, Carmen. Universidad Nacional Autónoma de México; México
dc.description.fil
Fil: Sanchez, Federico. Universidad Nacional Autónoma de México; México
dc.journal.title
Plant Cell
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/plcell/article/28/9/2326/6098387
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1105/tpc.15.01012
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