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dc.contributor.author
Cavello, Ivana Alejandra
dc.contributor.author
Contreras Esquivel, Juan Carlos
dc.contributor.author
Cavalitto, Sebastian Fernando
dc.date.available
2017-05-08T21:31:23Z
dc.date.issued
2014-08
dc.identifier.citation
Cavello, Ivana Alejandra; Contreras Esquivel, Juan Carlos; Cavalitto, Sebastian Fernando; Immobilization of a keratinolytic protease from Purpureocillium lilacinum on genipin activated-chitosan beads; Elsevier; Process Biochemistry; 49; 8; 8-2014; 1332-1336
dc.identifier.issn
1359-5113
dc.identifier.uri
http://hdl.handle.net/11336/16111
dc.description.abstract
Keratinase from Purpureocillium lilacinum LPSC # 876 was immobilized on chitosan beads using two different cross-linking agents: glutaraldehyde and genipin. For its immobilization certain parameters were optimized such as cross-linker concentration, activation time and activation temperature. Under optimum conditions, enzyme immobilization resulted to be 96 and 92.8% for glutaraldehyde and genipin, respectively, with an activity recovery reaching up to 81% when genipin was used. The immobilized keratinase showed better thermal and pH stabilities compared to the soluble form, retaining more than 85% of its activity at pH 11 and 74% at 50 °C after 1 h of incubation. The residual activity of immobilized keratinase remained more than 60% of its initial value after five hydrolytic cycles. The results in this study support that glutaraldehyde could be replaced by genipin as an alternative cross-linking eco-friendly agent for enzyme immobilization.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Keratinase
dc.subject
Immobilization
dc.subject
Genipin
dc.subject
Chitosan Beads
dc.subject
Hair Waste
dc.subject.classification
Bioprocesamiento Tecnológico, Biocatálisis, Fermentación
dc.subject.classification
Biotecnología Industrial
dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS
dc.title
Immobilization of a keratinolytic protease from Purpureocillium lilacinum on genipin activated-chitosan beads
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-05-05T20:08:29Z
dc.journal.volume
49
dc.journal.number
8
dc.journal.pagination
1332-1336
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Cavello, Ivana Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Cs.exactas. Centro de Investigación y Desarrollo En Fermentaciones Industriales; Argentina
dc.description.fil
Fil: Contreras Esquivel, Juan Carlos. Universidad Autónoma de Coahuila; México
dc.description.fil
Fil: Cavalitto, Sebastian Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Cs.exactas. Centro de Investigación y Desarrollo En Fermentaciones Industriales; Argentina
dc.journal.title
Process Biochemistry
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.procbio.2014.04.016
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1359511314002645


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