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dc.contributor.author
Vaňousová, Kateřina
dc.contributor.author
Beranová, Jana
dc.contributor.author
Fišer, Radovan
dc.contributor.author
Jemioła Rzemińska, Malgorzata
dc.contributor.author
Matyska Lišková, Petra
dc.contributor.author
Cybulski, Larisa Estefania
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dc.contributor.author
Strzałka, Kazimierz
dc.contributor.author
Konopásek, Ivo
dc.date.available
2022-07-01T12:48:30Z
dc.date.issued
2018-03
dc.identifier.citation
Vaňousová, Kateřina; Beranová, Jana; Fišer, Radovan; Jemioła Rzemińska, Malgorzata; Matyska Lišková, Petra; et al.; Membrane fluidization by alcohols inhibits DesK-DesR signalling in Bacillus subtilis; Elsevier Science; Biochimica et Biophysica Acta - Biomembranes; 1860; 3; 3-2018; 718-727
dc.identifier.issn
0005-2736
dc.identifier.uri
http://hdl.handle.net/11336/161035
dc.description.abstract
After cold shock, the Bacillus subtilis desaturase Des introduces double bonds into the fatty acids of existing membrane phospholipids. The synthesis of Des is regulated exclusively by the two-component system DesK/DesR; DesK serves as a sensor of the state of the membrane and triggers Des synthesis after a decrease in membrane fluidity. The aim of our work is to investigate the biophysical changes in the membrane that are able to affect the DesK signalling state. Using linear alcohols (ethanol, propanol, butanol, hexanol, octanol) and benzyl alcohol, we were able to suppress Des synthesis after a temperature downshift. The changes in the biophysical properties of the membrane caused by alcohol addition were followed using membrane fluorescent probes and differential scanning calorimetry. We found that the membrane fluidization induced by alcohols was reflected in an increased hydration at the lipid-water interface. This is associated with a decrease in DesK activity. The addition of alcohol mimics a temperature increase, which can be measured isothermically by fluorescence anisotropy. The effect of alcohols on the membrane periphery is in line with the concept of the mechanism by which two hydrophilic motifs located at opposite ends of the transmembrane region of DesK, which work as a molecular caliper, sense temperature-dependent variations in membrane properties.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ALCOHOLS
dc.subject
BACILLUS SUBTILIS
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COLD SHOCK
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MEMBRANE FLUIDITY
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MEMBRANE-ACTIVE COMPOUNDS
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TWO-COMPONENT SYSTEM
dc.subject.classification
Bioquímica y Biología Molecular
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Membrane fluidization by alcohols inhibits DesK-DesR signalling in Bacillus subtilis
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-06-21T18:35:51Z
dc.journal.volume
1860
dc.journal.number
3
dc.journal.pagination
718-727
dc.journal.pais
Países Bajos
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dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Vaňousová, Kateřina. Charles University; República Checa
dc.description.fil
Fil: Beranová, Jana. Charles University; República Checa
dc.description.fil
Fil: Fišer, Radovan. Charles University; República Checa
dc.description.fil
Fil: Jemioła Rzemińska, Malgorzata. Jagiellonian University; Polonia
dc.description.fil
Fil: Matyska Lišková, Petra. Charles University; República Checa
dc.description.fil
Fil: Cybulski, Larisa Estefania. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
dc.description.fil
Fil: Strzałka, Kazimierz. Jagiellonian University; Polonia
dc.description.fil
Fil: Konopásek, Ivo. Charles University; República Checa
dc.journal.title
Biochimica et Biophysica Acta - Biomembranes
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.bbamem.2017.12.015
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0005273617304078
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