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dc.contributor.author
Marsa, Silvana Mariel  
dc.contributor.author
Rossi, Franco Rubén  
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Valdez, Hugo Alberto  
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Gomez Casati, Diego Fabian  
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Zirulnik, Fanny  
dc.date.available
2017-05-08T20:29:31Z  
dc.date.issued
2015-06  
dc.identifier.citation
Marsa, Silvana Mariel; Rossi, Franco Rubén; Valdez, Hugo Alberto; Gomez Casati, Diego Fabian; Zirulnik, Fanny; Study of oxidative stress and genotoxicity by arsenic contamination in Glycine max. L; SCIENCEDOMAIN international; International Journal of Plant & Soil Science; 8; 1; 6-2015; 1-15  
dc.identifier.issn
2320-7035  
dc.identifier.uri
http://hdl.handle.net/11336/16091  
dc.description.abstract
Introduction: Volcanism is an important natural origin of Arsenic (As) contamination. Aims: Oxidative stress parameters, antioxidant defense system, the expression of transcripts involved in defense responses and the As genotoxicity were analyzed. Study Design: The plants were separated into two groups (Control and As-treated ones) until they were exposed to As (2.5 and 5 ppm) at day 10. Methodology: Biochemical, molecular and cytogenetic determinations were carried out. Results: Arsenic treated plants show growth abnormalities. The antioxidant defenses and oxidative parameters in roots and leaves showed different tissue-specific responses. Whereas leaf tissues display an active antioxidant defense, root cells are unable to produce defense molecules and show oxidative stress. Moreover, the analysis of antioxidant enzymes such as ascorbate peroxidase and superoxide dismutase, showed higher activity in leaves than in roots, except for isocitrate dehydrogenase. The expression of GR and MAPK decreased significantly in all the treatments during the time curve. ICDH showed an increase in its expression with 24 hours of treatment and falls at 72 hours compared to their controls. Genotoxicity assays showed a decrease of mitotic index (MI) and the presence of altered nucleus, in samples exposed for 72 hours. Conclusion: Our results suggest that the physiological, biochemical, molecular and cytogenetic behavior of the plants depends on the As concentration and the length of the exposure.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
SCIENCEDOMAIN international  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Arsenic  
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Soybean  
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Oxidative Stress  
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Antioxidant Defense  
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Bioquímica y Biología Molecular  
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Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Study of oxidative stress and genotoxicity by arsenic contamination in Glycine max. L  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2017-05-05T20:06:44Z  
dc.journal.volume
8  
dc.journal.number
1  
dc.journal.pagination
1-15  
dc.journal.pais
India  
dc.description.fil
Fil: Marsa, Silvana Mariel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina  
dc.description.fil
Fil: Rossi, Franco Rubén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "dr. Raul Alfonsin" (sede Chascomus) | Universidad Nacional de San Martin. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina  
dc.description.fil
Fil: Valdez, Hugo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "dr. Raul Alfonsin" (sede Chascomus) | Universidad Nacional de San Martin. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina  
dc.description.fil
Fil: Gomez Casati, Diego Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosinteticos y Bioquimicos. Universidad Nacional de Rosario. Facultad de Cs.bioquímicas y Farmaceuticas. Centro de Estudios Fotosinteticos y Bioquimicos; Argentina  
dc.description.fil
Fil: Zirulnik, Fanny. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina  
dc.journal.title
International Journal of Plant & Soil Science  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.9734/IJPSS/2015/18979  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://sciencedomain.org/abstract/10034