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dc.contributor.author
Godoy, Martín Sergio
dc.contributor.author
Castro Vazquez, Alfredo Juan
dc.contributor.author
Vega, Israel Aníbal
dc.date.available
2015-08-11T15:47:35Z
dc.date.issued
2013-06-20
dc.identifier.citation
Godoy, Martín Sergio; Castro Vazquez, Alfredo Juan; Vega, Israel Aníbal; Endosymbiotic and Host Proteases in the Digestive Tract of the Invasive Snail Pomacea canaliculata: Diversity, Origin and Characterization; Public Library Science; Plos One; 8; 6; 20-6-2013; 1-10
dc.identifier.issn
1932-6203
dc.identifier.uri
http://hdl.handle.net/11336/1606
dc.description.abstract
Digestive proteases of the digestive tract of the apple snail Pomacea canaliculata were studied. Luminal protease activity was found in the crop, the style sac and the coiled gut and was significantly higher in the coiled gut. Several protease bands and their apparent molecular weights were identified in both tissue extracts and luminal contents by gel zymography: (1) a 125 kDa protease in salivary gland extracts and in the crop content; (2) a 30 kDa protease throughout all studied luminal contents and in extracts of the midgut gland and of the endosymbionts isolated from this gland; (3) two proteases of 145 and 198 kDa in the coiled gut content. All these proteases were inhibited by aprotinin, a serine-protease inhibitor, and showed maximum activity between 30C and 35C and pH between 8.5 and 9.5. Tissue L-alanine-N-aminopeptidase activity was determined in the wall of the crop, the style sac and the coiled gut and was significantly higher in the coiled gut. Our findings show that protein digestion in P. canaliculata is carried out through a battery of diverse proteases originated from the salivary glands and the endosymbionts lodged in the midgut gland and by proteases of uncertain origin that occur in the coiled gut lumen.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Public Library Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Symbiosis
dc.subject
Serinproteases
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Aminopeptidaes
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Digestion
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Pomacea Canaliculata
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Snail
dc.subject.classification
Zoología, Ornitología, Entomología, Etología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Endosymbiotic and Host Proteases in the Digestive Tract of the Invasive Snail Pomacea canaliculata: Diversity, Origin and Characterization
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-30 10:35:44.97925-03
dc.journal.volume
8
dc.journal.number
6
dc.journal.pagination
1-10
dc.journal.pais
Estados Unidos
dc.journal.ciudad
San Francisco
dc.description.fil
Fil: Godoy, Martín Sergio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Mendoza. Instituto Histología y Embriología de Mendoza; Argentina; Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Fisiología; Argentina;
dc.description.fil
Fil: Castro Vazquez, Alfredo Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Mendoza. Instituto Histología y Embriología de Mendoza; Argentina; Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Fisiología; Argentina; Universidad Nacional de Cuyo, Instituto de Ciencias Básicas; Argentina;
dc.description.fil
Fil: Vega, Israel Aníbal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Mendoza. Instituto Histología y Embriología de Mendoza; Argentina; Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Fisiología; Argentina; Universidad Nacional de Cuyo, Instituto de Ciencias Básicas; Argentina;
dc.journal.title
Plos One
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/doi:10.1371/journal.pone.0066689
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.plosone.org/article/fetchObject.action?uri=info:doi/10.1371/journal.pone.0066689&representation=PDF
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