Mostrar el registro sencillo del ítem
dc.contributor.author
Julius, Peter
dc.contributor.author
Siyumbwa, Stepfanie N.
dc.contributor.author
Moonga, Phyllis
dc.contributor.author
Maate, Fred
dc.contributor.author
Kaile, Trevor
dc.contributor.author
Haynatski, Gleb
dc.contributor.author
Minhas, Veenu
dc.contributor.author
Snow, Jazmine
dc.contributor.author
Peterson, Kerstin
dc.contributor.author
Gihozo, Patience
dc.contributor.author
Streeter, Sam
dc.contributor.author
Kaur, Salan
dc.contributor.author
Evans, Annika
dc.contributor.author
Gonzalez, Daniela del Carmen
dc.contributor.author
Samwel, Kandali
dc.contributor.author
Kang, Guobin
dc.contributor.author
West, John T.
dc.contributor.author
Wood, Charles
dc.contributor.author
Angeletti, Peter C.
dc.date.available
2022-06-23T14:22:44Z
dc.date.issued
2022-04
dc.identifier.citation
Julius, Peter; Siyumbwa, Stepfanie N.; Moonga, Phyllis; Maate, Fred; Kaile, Trevor; et al.; Epstein–Barr Virus, but not human Papillomavirus, is associated with preinvasive and invasive ocular surface squamous neoplasias in Zambian patients; Frontiers Media; Frontiers in Oncology; 12; 4-2022; 1-15
dc.identifier.uri
http://hdl.handle.net/11336/160336
dc.description.abstract
Background: The etiopathogenesis of ocular surface squamous neoplasia (OSSN) is not fully understood. We assessed the frequency of oncogenic viruses in OSSN by immunohistochemistry (IHC) and polymerase chain reaction (PCR) for human papillomavirus (HPV), Epstein–Barr virus (EBV), Merkel cell polyomavirus (MCPyV), Kaposi sarcoma virus, and adenovirus. Cases from Zambia were prospectively enrolled using a cross-sectional study design between November 2017 and March 2020. Methods: Demographic and clinical data [age, sex, HIV status, antiretroviral therapy (ART) history, CD4 count, plasma viral load] and tumor biopsies were collected from 243 consenting patients. Tumor samples were bisected, and half was used for DNA isolation, while the other half was formalin fixed and paraffin embedded (FFPE) for histopathology analysis. The expressions of latent EBV nuclear antigen 1 (EBNA1), CDKN2A/p16INK4A (p16), and MCPyV large T-antigen (LT) were tested by IHC. Multiplex PCR was used to detect 16 HPV genotypes and four other DNA tumor viruses [Kaposi’s sarcomaassociated herpesvirus (KSHV), EBV, MCPyV, and adenovirus]. Relationships between HIV status, viral DNA and protein expression, and tumor grades were determined by statistical analysis. Results: OSSN tumors from patients were 29.6% preinvasive and 70.4% invasive. Patients presented with unilateral tumors that were 70.4% late stage (T3/T4). OSSN patients were HIV positive (72.8%). IHC on 243 FFPE biopsies resulted in the detection of EBNA1 (EBV), p16 high-risk HPV (HR-HPV), and MCPyV LT expression in 89.0%, 4.9%, and 0.0%, respectively. EBNA1 was expressed in all grades of preinvasive [corneaconjunctiva intraepithelial neoplasia (CIN)1, 100%; CIN2, 85.7%; CIN3, 95.8%; and carcinoma in situ (CIS), 83.8%] and in invasive (89.2%) OSSN. PCR on 178 samples detected EBV, HR-HPV, and MCPyV in 80.3%, 9.0%, and 13.5% of tumors, respectively. EBV was detected in all grades of preinvasive and invasive OSSN. EBV detection was associated with high HIV viral loads (p = 0.022). HR-HPV was detected in 0.0% CIN1, 0.0% CIN2, 5.6% CIN3, 13.0% CIS, and 7.0% invasive OSSN. Conclusions: Our findings of EBV DNA and EBNA1 protein in all the grades of preinvasive and especially invasive OSSN are consistent with a potential causal role for EBV in OSSN. A role of HPV in OSSN was not clearly established in this study.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Frontiers Media
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
OCULAR SURFACE SQUAMOUS NEOPLASIA
dc.subject
HUMAN PAPILLOMA VIRUS
dc.subject
EPSTEIN-BARR VIRUS
dc.subject
ZAMBIA
dc.subject
HUMAN IMMUNODEFICIENCY VIRUS
dc.subject.classification
Virología
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Epstein–Barr Virus, but not human Papillomavirus, is associated with preinvasive and invasive ocular surface squamous neoplasias in Zambian patients
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-06-21T18:32:12Z
dc.identifier.eissn
2234-943X
dc.journal.volume
12
dc.journal.pagination
1-15
dc.journal.pais
Suiza
dc.journal.ciudad
Lausana
dc.description.fil
Fil: Julius, Peter. School of Medicine. Department of Pathology and Microbiology; Zambia
dc.description.fil
Fil: Siyumbwa, Stepfanie N.. School of Medicine. Department of Pathology and Microbiology; Zambia
dc.description.fil
Fil: Moonga, Phyllis. University Teaching Hospital; Zambia
dc.description.fil
Fil: Maate, Fred. School of Medicine. Department of Pathology and Microbiology; Zambia
dc.description.fil
Fil: Kaile, Trevor. School of Medicine. Department of Pathology and Microbiology; Zambia
dc.description.fil
Fil: Haynatski, Gleb. University of Nebraska Medical Center. Department of Biostatistics; Estados Unidos
dc.description.fil
Fil: Minhas, Veenu. University of Nebraska Medical Center. Department of Biostatistics; Estados Unidos
dc.description.fil
Fil: Snow, Jazmine. Universidad de Nebraska - Lincoln; Estados Unidos
dc.description.fil
Fil: Peterson, Kerstin. Universidad de Nebraska - Lincoln; Estados Unidos
dc.description.fil
Fil: Gihozo, Patience. Universidad de Nebraska - Lincoln; Estados Unidos
dc.description.fil
Fil: Streeter, Sam. Universidad de Nebraska - Lincoln; Estados Unidos
dc.description.fil
Fil: Kaur, Salan. Universidad de Nebraska - Lincoln; Estados Unidos
dc.description.fil
Fil: Evans, Annika. Universidad de Nebraska - Lincoln; Estados Unidos
dc.description.fil
Fil: Gonzalez, Daniela del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina. Universidad de Nebraska - Lincoln; Estados Unidos
dc.description.fil
Fil: Samwel, Kandali. Ocean Road Cancer Institute; Tanzania
dc.description.fil
Fil: Kang, Guobin. Louisiana State University Health Science Center; Estados Unidos
dc.description.fil
Fil: West, John T.. Louisiana State University Health Science Center; Estados Unidos
dc.description.fil
Fil: Wood, Charles. Louisiana State University Health Science Center; Estados Unidos
dc.description.fil
Fil: Angeletti, Peter C.. Universidad de Nebraska - Lincoln; Estados Unidos
dc.journal.title
Frontiers in Oncology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fonc.2022.864066/full
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3389/fonc.2022.864066
Archivos asociados