Artículo
Na+ ATPase activities in chela muscle of the euryhaline crab Neohelice granulata: Differential response to environmental salinity
Fecha de publicación:
04/2009
Editorial:
Elsevier Science
Revista:
Journal of Experimental Marine Biology and Ecology
ISSN:
0022-0981
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The occurrence and characteristics of ouabain-insensitive Na+ ATPase activity and the response to environmental salinity of the coexistent Na+-K+ ATPase and ouabain-insensitive Na+ ATPase activities were studied in chela muscle of the euryhaline crab Neohelice (Chasmagnathus) granulata from Mar Chiquita coastal lagoon (Buenos Aires Province, Argentina). Chela muscle exhibited two ouabain-insensitive Na+ ATPase activities (a furosemide-insensitive and a furosemide-sensitive activity). I50 for ouabain-insensitive, furosemide-sensitive Na+ ATPase activity was about 1.4 mM. Both ouabain-insensitive, furosemide-insensitive and furosemide-sensitive Na+ ATPase activities were weakly affected by pH and showed Michaelis-Menten kinetics (Km = 0.021 and 0.224 mM, respectively). These characteristics appeared to be quite different from those previously described for Na+-K+ ATPase activity in chela muscle of this crab. Na+-K+ ATPase and ouabain-insensitive, furosemide-sensitive Na+ ATPase activities appeared to be sensitive to environmental salinity. In crabs acclimated to low salinity (10‰), a salinity at which N. granulata exhibits a strong hyperregulatory capacity, Na+-K+ ATPase activity was higher (117 ± 26 nmol Pi min- 1 mg prot- 1) than in 35‰ salinity (23 ± 6 nmol Pi min- 1 mg prot- 1) (a salinity at which this crab is osmoionoconforming). On the contrary, ouabain-insensitive, furosemide-sensitive Na+ ATPase activity was higher in 35‰ salinity (108 ±15 nmol Pi min- 1 mg prot- 1) than in crabs acclimated to 10‰ salinity (36 ± 11 nmol Pi min- 1 mg prot- 1). Ouabain-insensitive, furosemide-insensitive Na+ ATPase activity was not affected by acclimation of crabs to low salinity. The response to low salinity suggests that Na+-K+ ATPase could be a component of muscle regulatory mechanisms at the biochemical level secondary to hyperregulation whereas ouabain-insensitive, furosemide-sensitive activity appeared to be predominant upon osmoconforming conditions. The possible differential functional roles of Na+-K+ ATPase and ouabain-insensitive Na+ ATPase activities in muscle are discussed.
Palabras clave:
CRABS
,
MUSCLE
,
NA+ ATPASES
,
NEOHELICE GRANULATA
,
OSMOIONOREGULATION
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CCT - MAR DEL PLATA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - MAR DEL PLATA
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - MAR DEL PLATA
Articulos(IIMYC)
Articulos de INSTITUTO DE INVESTIGACIONES MARINAS Y COSTERAS
Articulos de INSTITUTO DE INVESTIGACIONES MARINAS Y COSTERAS
Citación
Pinoni, Silvina Andrea; Lopez Mañanes, Alejandra Antonia; Na+ ATPase activities in chela muscle of the euryhaline crab Neohelice granulata: Differential response to environmental salinity; Elsevier Science; Journal of Experimental Marine Biology and Ecology; 372; 1-2; 4-2009; 91-97
Compartir
Altmétricas