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dc.contributor.author
Suares, Alejandra Carolina
dc.contributor.author
Tapia, Cinthya Mariela
dc.contributor.author
González Pardo, María Verónica
dc.contributor.other
Hewinson, Martin
dc.contributor.other
Feet, James
dc.date.available
2022-06-14T03:16:10Z
dc.date.issued
2019
dc.identifier.citation
Antineoplastic effect of 1α,25(OH)2D3 in spheroids from endothelial cells transformed by Kaposi’s sarcoma-associated herpesvirus G protein coupled receptor; 21st Workshop on Vitamin D; Barcelona; España; 2018; 210-217
dc.identifier.issn
0960-0760
dc.identifier.uri
http://hdl.handle.net/11336/159634
dc.description.abstract
The Kaposi´s Sarcoma-associated Herpes virus G Protein-Coupled Receptor (vGPCR) is a key molecule in the pathogenesis of Kaposi?s sarcoma. Persistent expression and activity of vGPCR is required for NFB pathway activation and tumor maintenance in endothelial cells. We have previously demonstrated that 1α,25(OH)2D3 inhibits vGPCR cell growth, NFB activity and induces apoptosis in a VDR dependent manner. Many types of mammalian cells can aggregate and differentiate into 3-D multicellular spheroids (MCS) when cultured in suspension or a non adhesive environment. Compared to conventional mono-layer cultures (2D-cultures), MCS resemble real tissues better in terms of structural and functional properties. In this study we developed the method to obtain MCS from vGPCR cells in order to test whether MCS respond similar to 2D-cultures during 1α,25(OH)2D3. First, we found that vGPCR MCS started to form after 4 day-growth and reached a diameter of 200 µm at 12 day-growth, whereas cells without vGPCR expression remained in a starry shape. Secondly, vGPCR MCS size and architecture was analyzed during 1α,25(OH)2D3 (0-100 nM, 48 h) incubation. We found that MCS treated with 1 nM of 1α,25(OH)2D3 were compacted and began to disaggregate though no changes in their size were observed, whereas at the higher dose (100 nM) the architecture of MCS was broken. Furthermore, VDR mRNA expression increased significantly and this change was accompanied by a reduction of HIF-1α and an increase of VEGF, p21 and Bim mRNA expression. Finally, Results from Western blot analysis showed that 1α,25(OH)2D3 decreased ERK1/2 and Akt protein phosphorylation. In conclusion, these data have revealed that 1α,25(OH)2D3 inhibits vGPCR MCS proliferation and induces apoptosis similar to vGPCR 2D-cultures.Keywords: Spheroids, vGPCR cells, 1α,25(OH)2D3, anti-proliferative effects.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
dc.relation
https://www.sciencedirect.com/journal/the-journal-of-steroid-biochemistry-and-molecular-biology/special-issue/10H05QXM3J1
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
VITAMINA D
dc.subject
ENDOTHELIAL CELL
dc.subject
SPHEROIDS
dc.subject
ANTINEOPLASIC
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Medicina Básica
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Antineoplastic effect of 1α,25(OH)2D3 in spheroids from endothelial cells transformed by Kaposi’s sarcoma-associated herpesvirus G protein coupled receptor
dc.type
info:eu-repo/semantics/publishedVersion
dc.type
info:eu-repo/semantics/conferenceObject
dc.type
info:ar-repo/semantics/documento de conferencia
dc.date.updated
2022-04-21T17:18:05Z
dc.journal.volume
189
dc.journal.pagination
210-217
dc.journal.pais
España
dc.journal.ciudad
Barcelona
dc.description.fil
Fil: Suares, Alejandra Carolina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Cátedra de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
dc.description.fil
Fil: Tapia, Cinthya Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
dc.description.fil
Fil: González Pardo, María Verónica. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Cátedra de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/journal/the-journal-of-steroid-biochemistry-and-molecular-biology/special-issue/10H05QXM3J1
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/J.JSBMB.2019.03.026
dc.conicet.rol
Autor
dc.conicet.rol
Autor
dc.conicet.rol
Autor
dc.coverage
Internacional
dc.type.subtype
Congreso
dc.description.nombreEvento
21st Workshop on Vitamin D
dc.date.evento
2018-05-16
dc.description.ciudadEvento
Barcelona
dc.description.paisEvento
España
dc.type.publicacion
Journal
dc.description.institucionOrganizadora
University of East Anglia
dc.source.revista
The Journal of Steroid Biochemistry and Molecular Biology
dc.date.eventoHasta
2018-05-19
dc.type
Congreso
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