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dc.contributor.author
Centorbi, Hugo José
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dc.contributor.author
Silva, Humberto de Jesus
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dc.date.available
2022-06-10T12:57:08Z
dc.date.issued
2008-02
dc.identifier.citation
Centorbi, Hugo José; Silva, Humberto de Jesus; Slowly utilized carbon sources enhance botulinic specific toxicity by co-culture of Clostridium argentinense with the non-pathogen Pseudomonas mendocina; Springer; World Journal of Microbiology; 24; 2-2008; 1823-1828
dc.identifier.issn
0959-3993
dc.identifier.uri
http://hdl.handle.net/11336/159461
dc.description.abstract
The growth and botulinic toxin production of Clostridium argentinense G 89 HT in co-culture with Pseudomonas mendocina were studied using two culture systems with carbon sources of rapid and slow utilization. Growth with glucose in homogeneous co-culture presented a dual-phase progression, with the toxin produced in the slow-growing phase. The extended 50 h growth period of the second phase at low specific growth rate was attributed to the combined metabolization of glucose and a complex carbon source of the alginate type produced by P. mendocina under strongly reducing conditions. With dextrin, the co-culture grew at the lower specific growth rate (μ = 0.03 h−1) for a period lasting 80 h. This fully enhanced the production of toxin with a specific toxicity 2.5 times higher than with glucose in a homogeneous system and 10.7 higher than that of C. argentinense G 89 HT single culture. The heterogeneous co-culture obtained with a dialysis membrane physically separating both bacteria (thereby eliminating the metabolic interaction) produced the lowest levels of growth and toxin of all the cases analyzed.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
CARBON SOURCES
dc.subject
CLOSTRIDIUM ARGENTINENSE
dc.subject
CO-CULTURE-BOTULINIC TOXIN
dc.subject
PSEUDOMONAS MENDOCINA
dc.subject.classification
Biotecnología Industrial
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dc.subject.classification
Biotecnología Industrial
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dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS
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dc.title
Slowly utilized carbon sources enhance botulinic specific toxicity by co-culture of Clostridium argentinense with the non-pathogen Pseudomonas mendocina
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-06-09T13:36:36Z
dc.identifier.eissn
1573-0972
dc.journal.volume
24
dc.journal.pagination
1823-1828
dc.journal.pais
Alemania
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dc.journal.ciudad
Berlín
dc.description.fil
Fil: Centorbi, Hugo José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Laboratorio de Alimentos; Argentina
dc.description.fil
Fil: Silva, Humberto de Jesus. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Laboratorio de Alimentos; Argentina
dc.journal.title
World Journal of Microbiology
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s11274-008-9676-8
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1007/s11274-008-9676-8
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