Artículo
Microscopic and PCR-based detection of microsporidia spores in human stool samples
Mena, Cristian Javier
; Barnes, Andres Alberto; Castro, Gonzalo; Guasconi, Lorena
; Burstein, Verónica Liliana
; Beccacece, Ignacio
; Paulin, Patricia Catalina; Arneodo Larochette, Joel Demián
; Carnevale, Silvana
; Astudillo, Osvaldo Germán; Cervi, Laura Alejandra
; Theumer, Martín Gustavo
; Chiapello, Laura Silvina
Fecha de publicación:
06/2020
Editorial:
Asociación Argentina de Microbiología
Revista:
Revista Argentina de Microbiología
ISSN:
0325-7541
e-ISSN:
1851-7617
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Microsporidia are obligate intracellular fungi with a remarkable ability to infect a wide range of invertebrate and vertebrate hosts. Namely, Enterocytozoon bieneusi is the most frequently microsporidia reported worldwide, and mainly associated with chronic diarrhea and wasting syndrome in AIDS patients. Microscopy and PCR-based detection techniques are effective for diagnosis and identification of species and genotypes; however, these methods should be standardized in each laboratory. In this study, we performed microscopy and nested PCR techniques with PCR product sequencing to detect E. bieneusi in human stool samples. These techniques, if applied together, might prove useful for diagnosis and future epidemiological studies of intestinal microsporidiosis in Argentina.
Palabras clave:
MICROSPORIDIOSIS
,
ENTEROCYTOZOON BIENEUSI
,
NESTED PCR
,
TRICHROME STAIN
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Articulos(CIBICI)
Articulos de CENTRO DE INV.EN BIOQUI.CLINICA E INMUNOLOGIA
Articulos de CENTRO DE INV.EN BIOQUI.CLINICA E INMUNOLOGIA
Citación
Mena, Cristian Javier; Barnes, Andres Alberto; Castro, Gonzalo; Guasconi, Lorena; Burstein, Verónica Liliana; et al.; Microscopic and PCR-based detection of microsporidia spores in human stool samples; Asociación Argentina de Microbiología; Revista Argentina de Microbiología; 53; 2; 6-2020; 124-128
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