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dc.contributor.author
Gelaye, Woyneshet
dc.contributor.author
Williams, Nana Aba
dc.contributor.author
Kepha, Stella
dc.contributor.author
Messa Junior, Augusto
dc.contributor.author
Fleitas, Pedro Emanuel
dc.contributor.author
Marti Soler, Helena
dc.contributor.author
Damtie, Destaw
dc.contributor.author
Menkir, Sissay
dc.contributor.author
Krolewiecki, Alejandro Javier
dc.contributor.author
Van Lieshout, Lisette
dc.contributor.author
Enbiale, Wendemagegn
dc.date.available
2022-06-07T20:36:03Z
dc.date.issued
2021-03
dc.identifier.citation
Gelaye, Woyneshet; Williams, Nana Aba; Kepha, Stella; Messa Junior, Augusto; Fleitas, Pedro Emanuel; et al.; Performance evaluation of Baermann techniques: the quest for developing a microscopy reference standard for the diagnosis of Strongyloides stercoralis; Public Library of Science; Neglected Tropical Diseases; 15; 2; 3-2021; 1-13
dc.identifier.issn
1935-2735
dc.identifier.uri
http://hdl.handle.net/11336/159143
dc.description.abstract
Background: Soil-transmitted helminths (STH) are common in low and middle income countries where there is lack of access to clean water and sanitation. Effective diagnosis and treatment are essential for the control of STH infections. However, among STH parasites, Strongyloides stercoralis is the most neglected species, both in diagnostics and control strategies. Diagnostic methods cover different approaches, each with different sensitivities and specificities, such as serology, molecular techniques and microscopy based techniques. Of the later, the Baermann technique is the most commonly used procedure. In the literature, several ways have been described to perform the Baermann method, which illustrates the overall lack of a ‘(gold) reference standard’ method for the diagnosis of S. stercoralis infection. In this study we have evaluated the performance of three Baermann techniques in order to improve the reference standard for the microscopic diagnosis of S. stercoralis infection thereby facilitating individual case detection, mapping of the disease and proper evaluation of treatment responses. Methods/Principal findings: A community based cross sectional study was conducted at Zenzelima, Bahir Dar Zuria Ethiopia. A total of 437 stool samples were collected and analyzed by the following procedures: conventional Baermann (CB), modified Baermann (MB), and modified Baermann with charcoal pre-incubation (MBCI). The diagnostic sensitivity and Negative Predictive Value (NPV) of each technique was calculated using the combination of all the three techniques as a composite reference standard. Our result indicated that larvae of S. stercoralis were detected in 151 (34.6%) stool samples. The prevalence of S. stercoralis infection based on the three diagnostic methods was 9.6%, 8.0%, and 31.3% by CB, MB, and MBCI respectively. The sensitivity and NPV for CB, MB, and MBCI were 26.7% and 70.8%, 22.1% and 69.6%, and 87.0% and 93.2%, respectively. The MBCI showed significant difference (P- value = <0.001) in the sensitivity and NPV values when compared with CB and MB values. The agreement between CB, MB, and MBCI with the composite reference standard was 31.8%, 26.7%, 89.6%, respectively. Conclusion/Significance: Our results suggest the superior performance of MBCI. It is relatively easy to implement, simple to perform and comparatively cheaper. The CB is by far the commonly used method in routine diagnostic although this technique significantly underestimates the true burden of the disease and thereby contributing to the exclusion of S. stercoralis from the control strategies. Therefore, MBCI is recommended as a routine microscopy-based diagnostic test for S. stercoralis infection, particularly in settings where molecular procedures are not available.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Public Library of Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
BAERMANM
dc.subject
STRONGYLOIDES STERCORALIS
dc.subject
DIAGNOSTIC
dc.subject.classification
Parasitología
dc.subject.classification
Ciencias de la Salud
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Performance evaluation of Baermann techniques: the quest for developing a microscopy reference standard for the diagnosis of Strongyloides stercoralis
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-06-06T15:47:38Z
dc.journal.volume
15
dc.journal.number
2
dc.journal.pagination
1-13
dc.journal.pais
Estados Unidos
dc.journal.ciudad
San Francisco
dc.description.fil
Fil: Gelaye, Woyneshet. Bahir Dar University. College Of Medicine And Health; Etiopía
dc.description.fil
Fil: Williams, Nana Aba. Universidad de Barcelona; España
dc.description.fil
Fil: Kepha, Stella. Kenya Medical Research Institution; Kenia
dc.description.fil
Fil: Messa Junior, Augusto. Centro de Investigação em Saúde de Manhiça; Mozambique
dc.description.fil
Fil: Fleitas, Pedro Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina
dc.description.fil
Fil: Marti Soler, Helena. Universidad de Barcelona; España
dc.description.fil
Fil: Damtie, Destaw. Bahir Dar University. College of Science. Department of Biology; Etiopía
dc.description.fil
Fil: Menkir, Sissay. Bahir Dar University. College of Science. Department of Biology; Etiopía
dc.description.fil
Fil: Krolewiecki, Alejandro Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Universidad de Barcelona; España. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina
dc.description.fil
Fil: Van Lieshout, Lisette. Leiden University. Leiden University Medical Center; Países Bajos
dc.description.fil
Fil: Enbiale, Wendemagegn. Bahir Dar University, College Of Medicine And Health; Etiopía
dc.journal.title
Neglected Tropical Diseases
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0009076
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1371/journal.pntd.0009076
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