Mostrar el registro sencillo del ítem
dc.contributor.author
Martínez Noël, Giselle María Astrid
dc.contributor.author
Curatti, Leonardo
dc.contributor.author
Hernandez, Jose A.
dc.contributor.author
Rubio, Luis M.
dc.date.available
2017-05-02T20:56:31Z
dc.date.issued
2011-03
dc.identifier.citation
Martínez Noël, Giselle María Astrid; Curatti, Leonardo; Hernandez, Jose A.; Rubio, Luis M.; NifB and NifEN protein levels are regulated by ClpX2 under nitrogen fixation conditions in Azotobacter vinelandii; Wiley; Molecular Microbiology; 79; 5; 3-2011; 1182-1193
dc.identifier.issn
0950-382X
dc.identifier.uri
http://hdl.handle.net/11336/15903
dc.description.abstract
The major part of biological nitrogen fixation is catalysed by the molybdenum nitrogenase that carries at its active site the iron and molybdenum cofactor (FeMo-co). The nitrogen fixation (nif) genes required for the biosynthesis of FeMo-co are derepressed in the absence of a source of fixed nitrogen. The nifB gene product is remarkable because it assembles NifB-co, a complex cluster proposed to comprise a [6Fe-9S-X] cluster, from simpler [Fe-S] clusters common to other metabolic pathways. NifB-co is a common intermediate of the biosyntheses of the cofactors present in the molybdenum, vanadium and iron nitrogenases. In this work, the expression of the Azotobacter vinelandii nifB gene was uncoupled from its natural nif regulation to show that NifB protein levels are lower in cells growing diazotrophically than in cells growing at the expense of ammonium. A. vinelandii carries a duplicated copy of the ATPase component of the ubiquitous ClpXP protease (ClpX2), which is induced under nitrogen fixing conditions. Inactivation of clpX2 resulted in the accumulation of NifB and NifEN and a defect in diazotrophic growth, especially when iron was in short supply. Mutations in nifE, nifN and nifX or in nifA also affected NifB accumulation, suggesting that NifB susceptibility to degradation might vary during its catalytic cycle.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Azotobacter
dc.subject
Nitrogen Fixation
dc.subject
Femo-Co Biosynthesis
dc.subject
Clpx2
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.subject.classification
Biología Celular, Microbiología
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
NifB and NifEN protein levels are regulated by ClpX2 under nitrogen fixation conditions in Azotobacter vinelandii
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-04-24T20:43:34Z
dc.journal.volume
79
dc.journal.number
5
dc.journal.pagination
1182-1193
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Hoboken
dc.description.fil
Fil: Martínez Noël, Giselle María Astrid. Centro de Biotecnología y Genómica de Plantas; España
dc.description.fil
Fil: Curatti, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones En Biodiversidad y Biotecnología; Argentina
dc.description.fil
Fil: Hernandez, Jose A.. Midwestern University; Estados Unidos
dc.description.fil
Fil: Rubio, Luis M.. Centro de Biotecnología y Genómica de Plantas; España
dc.journal.title
Molecular Microbiology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2011.07540.x/abstract
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3104958/
Archivos asociados