Artículo
The pneumococcal two-component system SirRH is linked to enhanced intracellular survival of Streptococcus pneumoniae in influenza-infected pulmonary cells
Reinoso Vizcaíno, Nicolás Martín; Cian, Melina Beatriz
; Cortes, Paulo; Olivero, Nadia Belén
; Hernandez Morfa, Mirelys
; Piñas, German Eduardo
; Badapanda, Chandan; Rathore, Ankita; Perez, Daniel Roberto; Echenique, Jose Ricardo
Fecha de publicación:
01/2020
Editorial:
Public Library of Scienceplos@plos.org
Revista:
PLoS Pathogens
ISSN:
1553-7366
e-ISSN:
1553-7374
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The virus-bacterial synergism implicated in secondary bacterial infections caused by Streptococcus pneumoniae following infection with epidemic or pandemic influenza A virus (IAV) is well documented. However, the molecular mechanisms behind such synergism remain largely ill-defined. In pneumocytes infected with influenza A virus, subsequent infection with S. pneumoniae leads to enhanced pneumococcal intracellular survival. The pneumococcal two-component system SirRH appears essential for such enhanced survival. Through comparative transcriptomic analysis between the ΔsirR and wt strains, a list of 179 differentially expressed genes was defined. Among those, the clpL protein chaperone gene and the psaB Mn+2 transporter gene, which are involved in the stress response, are important in enhancing S. pneumoniae survival in influenza-infected cells. The ΔsirR, ΔclpL and ΔpsaB deletion mutants display increased susceptibility to acidic and oxidative stress and no enhancement of intracellular survival in IAV-infected pneumocyte cells. These results suggest that the SirRH two-component system senses IAV-induced stress conditions and controls adaptive responses that allow survival of S. pneumoniae in IAV-infected pneumocytes.
Palabras clave:
PNEUMOCOCCUS
,
INFLUENZA
,
INTERACTION
,
INTRACELLULAR
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Articulos(CIBICI)
Articulos de CENTRO DE INV.EN BIOQUI.CLINICA E INMUNOLOGIA
Articulos de CENTRO DE INV.EN BIOQUI.CLINICA E INMUNOLOGIA
Citación
Reinoso Vizcaíno, Nicolás Martín; Cian, Melina Beatriz; Cortes, Paulo; Olivero, Nadia Belén; Hernandez Morfa, Mirelys; et al.; The pneumococcal two-component system SirRH is linked to enhanced intracellular survival of Streptococcus pneumoniae in influenza-infected pulmonary cells; Public Library of Scienceplos@plos.org; PLoS Pathogens; 16; 8; 1-2020; 1-34
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