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dc.contributor.author
Quiroga, Evelina
dc.contributor.author
Priolo, Nora Silvia
dc.contributor.author
Marchese, Jose
dc.contributor.author
Barberis, Sonia Esther
dc.date.available
2022-06-03T11:56:56Z
dc.date.issued
2006-01
dc.identifier.citation
Quiroga, Evelina; Priolo, Nora Silvia; Marchese, Jose; Barberis, Sonia Esther; Behavior of Araujiain, a new cysteine phytoprotease, in organic media with low water content; Pontificia Universidad Católica de Valparaíso; Electronic Journal of Biotechnology; 9; 1; 1-2006; 18-25
dc.identifier.issn
0717-3458
dc.identifier.uri
http://hdl.handle.net/11336/158831
dc.description.abstract
In this paper we studied the effect of different organic solvents (1-octanol, trichloroethylene, ethanol, ethyl acetate, tetrahydrofuran, cyclohexane, propanone, acetonitrile, dichloromethane, chlorobenzene, N,N-dimethylformamide, acetophenone, diethyl ether, methanol, ethylene glycol and toluene) with low and constant water content on substrate preferences, thermostability and stability (caseinolytic activity retention after 4 h) of proteases of Araujia hortorum Fourn. (Asclepiadaceae). The stability of araujiain was high in N,N-dimethylformamide and ethanol at 40ºC, but decreased at higher temperature. Araujiain substrates preferences in buffer Tris-HCl (pH 8), ethylene glycol and N,N-dimethylformamide exhibited different patterns, but the enzyme showed a high preference by glutamine derivative in all cases. According to FTIR spectroscopy studies, araujiain changed its secondary structure and as a consequence, it also changed its substrate preferences. This enzyme showed lower α-helical character and greater β-sheet folding in buffer than in organic media. A larger amount of antiparallel β-sheet residues indicates the formation of tighter intermolecular hydrogen bonds and enzymatic aggregates. These facts could explain the higher esterolytic activities, the greater stability and good hydrolytic potential of araujiain in some organic media such as N,N-dimethylformamide.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Pontificia Universidad Católica de Valparaíso
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Araujia hortorum Fourn
dc.subject
Organic solvents
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Plant protease
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Substrate preferences
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Thermostability
dc.subject.classification
Ingeniería de Procesos Químicos
dc.subject.classification
Ingeniería Química
dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS
dc.title
Behavior of Araujiain, a new cysteine phytoprotease, in organic media with low water content
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-05-20T15:07:55Z
dc.journal.volume
9
dc.journal.number
1
dc.journal.pagination
18-25
dc.journal.pais
Chile
dc.journal.ciudad
Valparaíso
dc.description.fil
Fil: Quiroga, Evelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Farmacia. Laboratorio de Bromatología; Argentina
dc.description.fil
Fil: Priolo, Nora Silvia. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina
dc.description.fil
Fil: Marchese, Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Ciencias Fisico Matematicas y Naturales. Departamento de Fisica. Laboratorio de Ciencias de Superficies y Medios Porosos; Argentina
dc.description.fil
Fil: Barberis, Sonia Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Farmacia. Laboratorio de Bromatología; Argentina
dc.journal.title
Electronic Journal of Biotechnology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.ejbiotechnology.info/content/vol9/issue1/full/6/index.html
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.2225/vol9-issue1-fulltext-6
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