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dc.contributor.author
Lizasoain, Andrés  
dc.contributor.author
Mir, Daiana  
dc.contributor.author
Salvo, Matías  
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Bortagaray, Viviana  
dc.contributor.author
Masachessi, Gisela  
dc.contributor.author
Farías, Adrián Alejandro  
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Rodríguez Osorio, Nélida  
dc.contributor.author
Nates, Silvia Viviana  
dc.contributor.author
Victoria, Matías  
dc.contributor.author
Colina, Rodney  
dc.date.available
2022-06-02T04:14:32Z  
dc.date.issued
2021-08  
dc.identifier.citation
Lizasoain, Andrés; Mir, Daiana; Salvo, Matías; Bortagaray, Viviana; Masachessi, Gisela; et al.; First evidence of enterovirus A71 and echovirus 30 in Uruguay and genetic relationship with strains circulating in the South American region; Public Library of Science; Plos One; 16; 8; 8-2021; 1-19  
dc.identifier.issn
1932-6203  
dc.identifier.uri
http://hdl.handle.net/11336/158717  
dc.description.abstract
Human enteroviruses (EVs) comprise more than 100 types of coxsackievirus, echovirus, poliovirus and numbered enteroviruses, which are mainly transmitted by the faecal-oral route leading to diverse diseases such as aseptic meningitis, encephalitis, and acute flaccid paralysis, among others. Since enteroviruses are excreted in faeces, wastewater-based epidemiology approaches are useful to describe EV diversity in a community. In Uruguay, knowledge about enteroviruses is extremely limited. This study assessed the diversity of enteroviruses through Illumina next-generation sequencing of VP1-amplicons obtained by RT-PCR directly applied to viral concentrates of 84 wastewater samples collected in Uruguay during 2011–2012 and 2017–2018. Fifty out of the 84 samples were positive for enteroviruses. There were detected 27 different types belonging to Enterovirus A species (CVA2-A6, A10, A16, EV-A71, A90), Enterovirus B species (CVA9, B1-B5, E1, E6, E11, E14, E21, E30) and Enterovirus C species (CVA1, A13, A19, A22, A24, EV-C99). Enterovirus A71 (EV-A71) and echovirus 30 (E30) strains were studied more in depth through phylogenetic analysis, together with some strains previously detected by us in Argentina. Results unveiled that EV-A71 sub-genogroup C2 circulates in both countries at least since 2011–2012, and that the C1-like emerging variant recently entered in Argentina. We also confirmed the circulation of echovirus 30 genotypes E and F in Argentina, and reported the detection of genotype E in Uruguay. To the best of our knowledge this is the first report of the EV-A71 C1-like emerging variant in South-America, and the first report of EV-A71 and E30 in Uruguay.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Public Library of Science  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/  
dc.subject
ENTEROVIRUS A71  
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ECHOVIRUS 30  
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LLUMINA NEXT-GENERATION SEQUENCING  
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WASTEWATER SAMPLES  
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Virología  
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Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
First evidence of enterovirus A71 and echovirus 30 in Uruguay and genetic relationship with strains circulating in the South American region  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2022-05-12T16:50:05Z  
dc.identifier.eissn
1932-6203  
dc.journal.volume
16  
dc.journal.number
8  
dc.journal.pagination
1-19  
dc.journal.pais
Estados Unidos  
dc.journal.ciudad
San Francisco  
dc.description.fil
Fil: Lizasoain, Andrés. Universidad de la República. Centro Universitario del Litoral Norte. Centro Universitario de Salto; Uruguay  
dc.description.fil
Fil: Mir, Daiana. Universidad de la República. Centro Universitario del Litoral Norte. Centro Universitario de Salto; Uruguay  
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Fil: Salvo, Matías. Universidad de la República. Centro Universitario del Litoral Norte. Centro Universitario de Salto; Uruguay  
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Fil: Bortagaray, Viviana. Universidad de la República. Centro Universitario del Litoral Norte. Centro Universitario de Salto; Uruguay  
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Fil: Masachessi, Gisela. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina  
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Fil: Farías, Adrián Alejandro. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina  
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Fil: Rodríguez Osorio, Nélida. Universidad de la República. Centro Universitario del Litoral Norte. Centro Universitario de Salto; Uruguay  
dc.description.fil
Fil: Nates, Silvia Viviana. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología Dr. J. M. Vanella; Argentina  
dc.description.fil
Fil: Victoria, Matías. Universidad de la República. Centro Universitario del Litoral Norte. Centro Universitario de Salto; Uruguay  
dc.description.fil
Fil: Colina, Rodney. Universidad de la República. Centro Universitario del Litoral Norte. Centro Universitario de Salto; Uruguay  
dc.journal.title
Plos One  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0255846  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1371/journal.pone.0255846