Artículo
Detection of Yersinia spp. in meat products by enrichment culture, immunomagnetic separation and nested PCR
Lucero Estrada, Cecilia Stella Marys
; Velázquez, Lidia del Carmen; Favier, Gabriela Isabel
; Di Genaro, Maria Silvia
; Escudero, María Esther
Fecha de publicación:
05/2012
Editorial:
Elsevier
Revista:
Food Microbiology
ISSN:
0740-0020
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The prevalence of Yersinia enterocolitica in meat products was assessed by four methods: cold enrichment in trypticase soy broth (A), enrichment in modified Rappaport broth at 25 C (B), concentration by immunomagnetic separation (C) and yadA nested PCR (D). Furthermore, the pathogenic potentials of the isolates were established by phenotypic and genotypic tests, and their genomic relationships were determined by pulsed-field gel electrophoresis (PFGE). A total of 238 samples were collected at retail level in the city of San Luis, Argentina, during the period 2007e2008. The highest Yersinia prevalence in meat products was observed by method D (92 positive samples), followed by methods A (13 positive samples) and C (5 positive samples); however, no isolation was obtained by method B. Fourteen Y. enterocolitica and 4 Yersinia intermedia strains were recovered by culture. All Y. enterocolitica 2/O:9 strains gave results related to virulence by phenotypic tests and exhibited the genotype virF þ myfA þ ail þ ystA þ . Two biotype 1A strains showed a genotype virF myfA ail þ ystA þ ystB þ . The 14 Y. enterocolitica strains isolated during this work plus one reference strain were separated into 11 genomic types by PFGE. This genomic heterogeneity of the isolates shows the diversity of Y. enterocolitica strains in our region. It is the first time that IMS was used to search Y. enterocolitica strains from naturally contaminated meat products.
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Articulos(CCT - SAN LUIS)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SAN LUIS
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SAN LUIS
Citación
Lucero Estrada, Cecilia Stella Marys; Velázquez, Lidia del Carmen; Favier, Gabriela Isabel; Di Genaro, Maria Silvia; Escudero, María Esther; Detection of Yersinia spp. in meat products by enrichment culture, immunomagnetic separation and nested PCR; Elsevier; Food Microbiology; 30; 1; 5-2012; 157-163
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