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dc.contributor.author
Peralta, Mariana Andrea  
dc.contributor.author
Calise, Maximiliano  
dc.contributor.author
Fornari, M. Cecilia  
dc.contributor.author
Ortega, María Gabriela  
dc.contributor.author
Diez, Roberto A.  
dc.contributor.author
Cabrera, Jose Luis  
dc.contributor.author
Perez, Cristina  
dc.date.available
2017-04-28T18:54:19Z  
dc.date.issued
2012-05  
dc.identifier.citation
Peralta, Mariana Andrea; Calise, Maximiliano; Fornari, M. Cecilia; Ortega, María Gabriela; Diez, Roberto A.; et al.; A Prenylated Flavanone from Dalea elegans Inhibits Rhodamine 6 G Efflux and Reverses FluconazoleResistance in Candida albicans; Georg Thieme Verlag Kg; Planta Medica; 78; 10; 5-2012; 981-987  
dc.identifier.issn
0032-0943  
dc.identifier.uri
http://hdl.handle.net/11336/15846  
dc.description.abstract
In previous studies, 2′,4′-dihydroxy-5′-(1′′′,1′′′-dimethylallyl)-6-prenylpinocembrin, a prenylated flavonoid isolated from Dalea elegans roots, showed activity against multiresistant Staphylococcus aureus and Candida albicans, as well as an uncoupling effect on mitochondria and antioxidant activity. The aim of this study was to evaluate the inhibitory effects of 2′,4′-dihydroxy-5′-(1′′′,1′′′-dimethylallyl)-6-prenylpinocembrin and fluconazole on the efflux of rhodamine 6 G in azole-resistant C. albicans 12–99 that expresses multidrug transporters Cdr1p, Cdr2p, and Mdr1p. The effect of fluconazole and 2′,4′-dihydroxy-5′-(1′′′,1′′′-dimethylallyl)-6-prenylpinocembrin on rhodamine 6 G efflux was assessed in both azole-sensitive and azole-resistant C. albicans. Between 1 and 1000 µM, 2′,4′-dihydroxy-5′-(1′′′,1′′′-dimethylallyl)-6-prenylpinocembrin inhibited rhodamine 6 G efflux only in azole-resistant C. albicans 12–99 in a concentration-dependent manner (IC50 = 119 µM); a competitive effect was observed. It also showed selectivity of action in comparison with other flavanones (6-prenylpinocembrin, isolated from aerial parts of D. elegans, pinocembrin, naringenin, and hesperetin, all at 250 µM). To check the possible implications of the inhibition of azole efflux on cell growth, antifungal assays were conducted. Minimal inhibitory concentration values were 150 µM for 2′,4′-dihydroxy-5′-(1′′′,1′′′-dimethylallyl)-6-prenylpinocembrin and higher than 400 µM for fluconazole. The combination of both compounds at either inhibitory or subinhibitory concentrations was significantly more effective than each compound separately. Minimal inhibitory concentration for fluconazole decreased by more than 400 times in the presence of 100 µM 2′,4′-dihydroxy-5′-(1′′′,1′′′-dimethylallyl)-6-prenylpinocembrin, reversing azole resistance and giving values similar to those of azole-sensitive C. albicans. These data are consistent with a dual action of 2′,4′-dihydroxy-5′-(1′′′,1′′′-dimethylallyl)-6-prenylpinocembrin: direct antifungal effect on azole-resistant C. albicans 12–99 and inhibition of azole transporters, which results in reversion of fluconazole resistance.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Georg Thieme Verlag Kg  
dc.rights
info:eu-repo/semantics/restrictedAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Prenylated Flavonoid  
dc.subject
Dalea Elegans  
dc.subject
Fabaceae  
dc.subject
Rhodamine Efflux Inhibition  
dc.subject.classification
Otras Ciencias Biológicas  
dc.subject.classification
Ciencias Biológicas  
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS  
dc.title
A Prenylated Flavanone from Dalea elegans Inhibits Rhodamine 6 G Efflux and Reverses FluconazoleResistance in Candida albicans  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2017-04-28T17:12:21Z  
dc.journal.volume
78  
dc.journal.number
10  
dc.journal.pagination
981-987  
dc.journal.pais
Alemania  
dc.description.fil
Fil: Peralta, Mariana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina  
dc.description.fil
Fil: Calise, Maximiliano. Universidad de Buenos Aires. Facultad de Medicina. Cátedra de Farmacología; Argentina  
dc.description.fil
Fil: Fornari, M. Cecilia. Universidad de Buenos Aires. Facultad de Medicina. Cátedra de Farmacología; Argentina  
dc.description.fil
Fil: Ortega, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina  
dc.description.fil
Fil: Diez, Roberto A.. Universidad de Buenos Aires. Facultad de Medicina. Cátedra de Farmacología; Argentina  
dc.description.fil
Fil: Cabrera, Jose Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina  
dc.description.fil
Fil: Perez, Cristina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina  
dc.journal.title
Planta Medica  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1055/s-0031-1298627  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.thieme-connect.de/DOI/DOI?10.1055/s-0031-1298627