Stability and fragmentation versus demographic expansion: different phylogeographic patterns in closely related sympatric legumes (Senna) from arid and semiarid zones of mid-latitude South America

Abstract

This is the first comparative research in which phylogeographical patterns and present and paleoclimatic distribution were investigated in desert plants that inhabit the Argentinian Monte and Chaco biogeographic provinces. We investigated two closely related and partially sympatric species of legume shrubs, Senna aphylla and S. pachyrrhiza, to contribute to understand the evolutionary history of arid plants and the impact of Quaternary climatic oscillations in these regions. We carried out phylogeographic analyses using cDNA accD-psa1 and rpL32-trnL sequences and reconstructed paleodistribution by ecological niche modelling (ENM). In S. aphylla, that inhabits northern areas of the Monte, the haplotype network together with demographic and spatial analyses and ENM indicated that the populations have remained demographic and spatially stable and would have undergone fragmentation in intermountain valleys and bolsons. In S. pachyrrhiza, that distributes mainly along the Monte and southwestern Dry Chaco, the haplotype network presented a ?star-like? topology and demographic analyses and ENM supported a recent demographic expansion but no range expansion. Both legumes have diversified approximately 2.5 Myr, survived Quaternary glaciations and have responded differently to climatic changes, complex topography and environmental heterogeneity. Then, the complex geological and climatic history of arid lands in mid-latitude South America provides different scenarios that promoted a mosaic of phylogeographical patterns.