Evento
Bone-Marrow Derived Dendritic Cells From Toxoplasma Gondii Chronically Infected Mice Exhibit Alterations In Monoclonal And Polyclonal T Cell Priming
Perrone Sibilia, Matias
; Katan Piñeiro, Joel; Soto, Ariadna Soledad
; Arcon, Nadia
; Sánchez, Vanesa Roxana
; Martín, Valentina
; Fenoy, I; Goldman, Alejandra
Colaboradores:
Curino, Alejandro; Maccioni, Mariana; Schaiquevich, Paula; Duran, Hebe
Tipo del evento:
Reunión
Nombre del evento:
LXVI Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXIX Anual de la Sociedad Argentina de Inmunología; LIII Reunión Anual de La Asociación Argentina de Farmacología Experimental y XI Reunión Anual de La Asociación Argentina de Nanomedicinas
Fecha del evento:
17/11/2021
Institución Organizadora:
Sociedad Argentina de Investigación Clínica;
Sociedad Argentina de Inmunología;
Asociación Argentina de Farmacología Experimental;
Asociación Argentina de Nanomedicinas;
Título de la revista:
Medicina (Buenos Aires)
Editorial:
Fundación Revista Medicina
ISSN:
1669-9106
e-ISSN:
0025-7680
Idioma:
Inglés
Clasificación temática:
Resumen
Rationale: We previously showed that splenocytes from chronic T. gondii infected mice have a diminished capacity to activate and differentiate OVA-specific Th1 and Th2 cells. Moreover, BMDCs from infected animals presented phenotypic alterations as shown by increases in CD80 and CD86 maturation markers and fewer secretion levels of IL-6 and IL-10, with no differences in IL-12. To extend these previous results, herein, we studied the ability of BMDC from chronically infected mice to activate and differentiate effector T cells. Methods: Bone-marrow derived dendritic cells (BMDCs) were obtained from naive and chronically infected mice, by culturing bone-marrow precursors for nine days with GM-CSF-conditioned medium. Afterward, BMDC were fed with OVA and matured during 18h with LPS. Subsequently, BMDC were cultured with DO11.10 OVA-specific CD4+T cells. Also, a mixed lymphocyte reaction (MLR) was performed by co-culturing BMDC with naive C57BL/6 mice splenocytes. Results: OVA specific CD4+ T cells co-cultured with BMDCs from infected mice showed lower levels of IFN-γ and IL-5 and increased levels of IL-10 (p<0.05). When analyzing polyclonal T cell responses in MLR assays, T lymphocytes incubated with BMDCs from infected mice showed decreased secretion of IFN-γ and IL-10 (p<0.01). No significant differences were observed for Th2 cytokines. Conclusion: The data obtained with BMDCs show that mice chronically infected with T. gondii present alterations of bone-marrow precursors. Interestingly, the diminished Th1/Th2 profiles observed in antigen specific co-culture assays are in line with results previously observed in splenocytes from T. gondii infected mice. These results suggest that infection with T. gondii results in long-lasting alterations in hematopoietic cells that could be involved in the lower susceptibility to developing allergic and autoimmune disorders.
Palabras clave:
Toxoplasmosis
,
Modulacion respuesta inmune
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Eventos(OCA PQUE. CENTENARIO)
Eventos de OFICINA DE COORDINACION ADMINISTRATIVA PQUE. CENTENARIO
Eventos de OFICINA DE COORDINACION ADMINISTRATIVA PQUE. CENTENARIO
Citación
Bone-Marrow Derived Dendritic Cells From Toxoplasma Gondii Chronically Infected Mice Exhibit Alterations In Monoclonal And Polyclonal T Cell Priming; LXVI Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXIX Anual de la Sociedad Argentina de Inmunología; LIII Reunión Anual de La Asociación Argentina de Farmacología Experimental y XI Reunión Anual de La Asociación Argentina de Nanomedicinas; Buenos Aires; Argentina; 2021; 113-113
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