Artículo
Sex chromosomes in the Vizcacheras’ White-lipped frog, Leptodactylus bufonius (Anura, Leptodactylidae)
Schneider, Rosio Gabriela
; Ferro, Juan Martín
; Reinko, Ivana Noelia; Boeris, Juan Martín
; Cardozo, Dario Elbio
; Baldo, Juan Diego
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Fecha de publicación:
06/2021
Editorial:
Academia Brasilera de Ciencias
Revista:
Anais da Academia Brasileira de Ciências
ISSN:
0001-3765
e-ISSN:
1678-2690
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Cytogenetic analyses were performed on specimens of Leptodactylus bufonius from different localities in Argentina. Mitotic chromosomes were studied with Giemsa and differential staining techniques (Ag-NOR, C-banding, and CMA3 /DAPI) and fl uorescence in situ hybridization with the 18S DNAr probe. All specimens showed karyotypes with 2n = 2x = 22 and FN = 44. Secondary constrictions were present in the long arm of chromosome pair 8, coincident with Ag-NOR and hybridization signals of the 18S DNAr probe. The C-banding technique evidenced an important amount of heterochromatin with a sex-linked pericentromeric band in the short arm of chromosome pair 4. This heterochromatic band was heteromorphic in males but present in both homologues of females, and it was CMA3 positive (DAPI negative) at fl uorescence staining. The occurrence of heteromorphic XY sex chromosomes in L. bufonius is the second known case in Leptodactylus and the fi fth within the speciose family Leptodactylidae.
Palabras clave:
C-BANDING
,
CYTOGENETICS
,
HETEROCHROMATIN
,
HETEROMORPHISM
,
XY
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(IBS)
Articulos de INSTITUTO DE BIOLOGIA SUBTROPICAL
Articulos de INSTITUTO DE BIOLOGIA SUBTROPICAL
Articulos(IDEAUS)
Articulos de INSTITUTO DE DIVERSIDAD Y EVOLUCION AUSTRAL
Articulos de INSTITUTO DE DIVERSIDAD Y EVOLUCION AUSTRAL
Citación
Schneider, Rosio Gabriela; Ferro, Juan Martín; Reinko, Ivana Noelia; Boeris, Juan Martín; Cardozo, Dario Elbio; et al.; Sex chromosomes in the Vizcacheras’ White-lipped frog, Leptodactylus bufonius (Anura, Leptodactylidae); Academia Brasilera de Ciencias; Anais da Academia Brasileira de Ciências; 93; 2; 6-2021; 1-12
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