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dc.contributor.author
Loto, Flavia del Valle
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Romero, Cintia Mariana
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Carrizo, Alfonso Emanuel
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Baigori, Mario Domingo
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Pera, Licia Maria
dc.date.available
2015-08-07T14:15:09Z
dc.date.issued
2013-11
dc.identifier.citation
Loto, Flavia del Valle; Romero, Cintia Mariana; Carrizo, Alfonso Emanuel; Baigori, Mario Domingo; Pera, Licia Maria; Hydrolytic and synthetic activities of esterases produced by Bacillus sp. A60 isolated from an oil contaminated soil; Academic Journals; African Journal of Biotechnology; 12; 11-2013; 6625-6631
dc.identifier.issn
1684-5315
dc.identifier.uri
http://hdl.handle.net/11336/1569
dc.description.abstract
A novel esterase producer strain named Bacillus sp. A60 was isolated from a soil sample contaminated with hydrocarbons. It was found to belong to Bacillus subtilis species through morphological, biochemical and 16S rRNA gene sequence analyses. This strain which can tolerate 15% (w/v) NaCl and growth at 55°C, produced an interesting esterase activity in Luria-Bertani medium. Two different molecular weight esterase activities were detected in zymographic assays. Culture supernatant and whole cells showed specific hydrolytic activities of 2.67 ± 0.11 U/mg of protein and 7.07 ± 0.09 U/mg of dry weight, respectively. Concerning ethyl acetate production, conversions of 88.00 ± 0 and 55.58 ± 0.78% were obtained with culture supernatant entrapped in polyacrylamine gel and whole cells, respectively. In addition, the effect of different concentration of LB medium components on both growth and extracellular esterase hydrolytic activity was also discussed.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Academic Journals
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
BACILLUS SP. A60
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ESTERASES
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HYDROLYTIC ACTIVITY
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SYNTHETIC ACTIVITY
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ETHYL ACETATE
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Biología Celular, Microbiología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Hydrolytic and synthetic activities of esterases produced by Bacillus sp. A60 isolated from an oil contaminated soil
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-30 10:35:44.97925-03
dc.journal.volume
12
dc.journal.pagination
6625-6631
dc.journal.pais
Estados Unidos
dc.journal.ciudad
New York
dc.description.fil
Fil: Loto, Flavia del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil
Fil: Romero, Cintia Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil
Fil: Carrizo, Alfonso Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil
Fil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil
Fil: Pera, Licia Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.journal.title
African Journal of Biotechnology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.5897/AJB2013.12123
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info:eu-repo/semantics/altIdentifier/url/http://www.academicjournals.org/article/article1385139810_Loto%20et%20al.pdf