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dc.contributor.author
Santiago Valtierra, Florencia Ximena  
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Peñalva, Daniel Alejandro  
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Luquez, Jessica Mariela  
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Aveldaño, Marta Isabel  
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Reyes, Juan Guillermo  
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Oresti, Gerardo Martin  
dc.date.available
2022-05-09T11:48:32Z  
dc.date.issued
2020  
dc.identifier.citation
Active calcium mobilization from a thapsigargin-sensitive pool by free 32.5n6 in spermatids; LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology (SAIB); XV Annual Meeting Argentinean Society for General Microbiology (SAMIGE); Mendoza; Argentina; 2020; 1-7  
dc.identifier.issn
0327-9545  
dc.identifier.uri
http://hdl.handle.net/11336/156881  
dc.description.abstract
In their free form, long chain (C18–22) polyunsaturated fatty acids (PUFA), especially 20:4n−6, can modify calcium homeostasis in male germ cells. These cells also contain unusual n-6 very-long-chain (VLC) PUFA (28:4, 30:5, and 32:5), in non-hydroxy (n-V) and 2-hydroxy (h-V) forms, in their membrane sphingolipids. Potential biological roles of VLCPUFA, as free fatty acids (FFA), in the physiology of male germ cells are unknown. In this study we explored the ability of n-V FFA, and their h-V counterparts, to modify the intracellular calcium homeostasis in rat spermatids. After obtaining the n-V and h-V FFA from testicular sphingomyelin, their natural source, each group was separately added to spermatids suspensions at an 8 µM concentration. The n-V FFA increased the intracellular calcium concentration ([Ca2+]i) in spermatids several fold more intensely than did the h-V FFA. After isolating the components of n-V and h-V mixtures by HPLC to study the effect of each VLCPUFA, free n-32:5 was found to be the most active in increasing the [Ca2+]i, followed by n-30:5, while h-32:5 augmented it only slightly. In addition to fatty acid-specific, the response was dose-dependent. The rates of [Ca2+]i upsurge were independent of the presence of extracellular calcium. Pretreatment with thapsigargin inhibited the effect of n-32:5, suggesting that this FFA promotes the release of Ca2+ from intracellular calcium stores, mainly the endoplasmic reticulum. The n-V FFA did not seem to exert their effects through the G protein-coupled receptor GPR120, a putative receptor for free PUFA, as they occurred in the presence of a GPR120 inhibitor. Ceramides containing the same fatty acids did not modify [Ca2+]i, thus the observed [Ca2+]i increases may be attributed to the FFA themselves. The possibility that they occur after VLC-FFAs are converted into other bioactive compounds remains to be investigated. Our results revealed a biological activity of VLCPUFA that suggests a physiological role for these fatty acids. As VLCPUFA-mediated Ca2+ rises occurred in spermatids, they may activate Ca2+ signaling pathways with specific functional targets in germ cells differentiation.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Tech Science Press  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
FREE VLCPUFA  
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CALCIUM HOMEOSTASIS  
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GERM CELLS  
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SPERMATOGENESIS  
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Bioquímica y Biología Molecular  
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Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Active calcium mobilization from a thapsigargin-sensitive pool by free 32.5n6 in spermatids  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.type
info:eu-repo/semantics/conferenceObject  
dc.type
info:ar-repo/semantics/documento de conferencia  
dc.date.updated
2022-04-21T17:24:31Z  
dc.identifier.eissn
1667-5746  
dc.journal.volume
45  
dc.journal.pagination
1-7  
dc.journal.pais
Argentina  
dc.journal.ciudad
Mendoza  
dc.description.fil
Fil: Santiago Valtierra, Florencia Ximena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina  
dc.description.fil
Fil: Peñalva, Daniel Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina  
dc.description.fil
Fil: Luquez, Jessica Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina  
dc.description.fil
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina  
dc.description.fil
Fil: Reyes, Juan Guillermo. Pontificia Universidad Católica de Chile; Chile  
dc.description.fil
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.saib.org.ar/index.php?q=node/562  
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Autor  
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Autor  
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Autor  
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Nacional  
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Reunión  
dc.description.nombreEvento
LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology (SAIB); XV Annual Meeting Argentinean Society for General Microbiology (SAMIGE)  
dc.date.evento
2020-11-02  
dc.description.ciudadEvento
Mendoza  
dc.description.paisEvento
Argentina  
dc.type.publicacion
Journal  
dc.description.institucionOrganizadora
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular  
dc.description.institucionOrganizadora
Sociedad Argentina de Microbiología General  
dc.source.revista
Biocell  
dc.date.eventoHasta
2020-11-05  
dc.type
Reunión